[3dem] Excessive drift/motion on 2nm ultrathin carbon coated grids

[David Michael Belnap]

Guobin,

I have used those grids for tomography.  I haven’t noticed any problems tilting the grids 60 degrees.

David


From: Guobin Hu <gb_hu at yahoo.com>
Date: Wednesday, November 30, 2022 at 10:54 AM
To: David Michael Belnap <David.Belnap at utah.edu>
Cc: 3DEM Mailing List <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] Excessive drift/motion on 2nm ultrathin carbon coated grids

Hi David and all the colleagues who replied off-line,

Thank you all for sharing your experience!

The feedbacks I have received so far suggest ultrathin carbon coated grids do not lead to excessive drift/motion unless the grid is tilted for data acquisition. Interestingly, our microscope did not seem to be the cause of the excessive drift/motion because no similar drift/motion was observed with uncoated grids.  Tests on thick carbon regions of coated grids did not show similar drift/motion either. We have ordered new ultrathin carbon coated grids. I will post updates once we use the new grids.

Feedbacks are still welcome and will be appreciated.

Best,
Guobin


Sent from my iPhone, please excuse typos


On Nov 22, 2022, at 1:33 PM, David Michael Belnap <David.Belnap at utah.edu> wrote:
Guobin,

I have used 671-200-Cu grids from Ted Pella several times without problems.  I don’t recall ever having drift problems.  I was imaging virus or virus-like particles on these grids.  671-200-Cu grids are Quantifoil R2/1 grids coated with 2 nm C.

David

=======================================
David M. Belnap
Electron Microscopy Core Laboratory
Department of Biochemistry
School of Biological Sciences
2501 SMBB
University of Utah
Salt Lake City, Utah 84112 USA
Phone  801.585.1242
FAX  801.587.3077
David.Belnap at utah.edu<mailto:David.Belnap at utah.edu>
=======================================



From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Guobin Hu <gb_hu at yahoo.com>
Date: Friday, November 18, 2022 at 8:22 PM
To: 3DEM Mailing List <3dem at ncmir.ucsd.edu>
Subject: [3dem] Excessive drift/motion on 2nm ultrathin carbon coated grids

Dear all,

I am collecting data with 2nm ultrathin carbon coated Quantifoil holy grids and observing excessive/abnormal drift/motion in images which I did not see with regular Quantifoil grids or other type of grids. There is no indication of the microscope as the cause of drift/motion. I am curious if anyone else has also observed the similar motion/drift with 2nm ultrathin carbon coated grids. FFTs w/ and w/o  drift/motion are attached as references. Any feedback insight will be appreciated.

Thanks,
Guobin




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