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<p class="MsoNormal"><span style="font-family:"Georgia",serif">Guobin,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif">I have used those grids for tomography. I haven’t noticed any problems tilting the grids 60 degrees.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif">David<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black">Guobin Hu <gb_hu@yahoo.com><br>
<b>Date: </b>Wednesday, November 30, 2022 at 10:54 AM<br>
<b>To: </b>David Michael Belnap <David.Belnap@utah.edu><br>
<b>Cc: </b>3DEM Mailing List <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>Re: [3dem] Excessive drift/motion on 2nm ultrathin carbon coated grids<o:p></o:p></span></p>
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<p class="MsoNormal">Hi David and all the colleagues who replied off-line,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Thank you all for sharing your experience!<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">The feedbacks I have received so far suggest ultrathin carbon coated grids do not lead to excessive drift/motion unless the grid is tilted for data acquisition. Interestingly, our microscope did not seem to be the cause of the excessive
drift/motion because no similar drift/motion was observed with uncoated grids. Tests on thick carbon regions of coated grids did not show similar drift/motion either. We have ordered new ultrathin carbon coated grids. I will post updates once we use the new
grids.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Feedbacks are still welcome and will be appreciated.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Best,<o:p></o:p></p>
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<p class="MsoNormal">Guobin<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><o:p> </o:p></p>
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<p class="MsoNormal">Sent from my iPhone, please excuse typos<o:p></o:p></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt">On Nov 22, 2022, at 1:33 PM, David Michael Belnap <David.Belnap@utah.edu> wrote:<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-family:"Georgia",serif">Guobin,</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif">I have used 671-200-Cu grids from Ted Pella several times without problems. I don’t recall ever having drift problems. I was imaging virus or virus-like particles on these grids. 671-200-Cu grids
are Quantifoil R2/1 grids coated with 2 nm C.</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif">David</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif"> </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.5pt;font-family:"Georgia",serif;color:black">=======================================<br>
David M. Belnap<br>
Electron Microscopy Core Laboratory<br>
Department of Biochemistry<br>
School of Biological Sciences<br>
2501 SMBB<br>
University of Utah<br>
Salt Lake City, Utah 84112 USA<br>
Phone 801.585.1242<br>
FAX 801.587.3077<br>
</span><span style="font-family:"Georgia",serif;color:black"><a href="mailto:David.Belnap@utah.edu" target="_blank" title="mailto:David.Belnap@utah.edu"><span style="font-size:11.5pt;color:#0563C1">David.Belnap@utah.edu</span></a></span><span style="font-size:11.5pt;font-family:"Georgia",serif;color:black"><br>
=======================================</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-family:"Georgia",serif"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Georgia",serif"> </span><o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Guobin Hu <gb_hu@yahoo.com><br>
<b>Date: </b>Friday, November 18, 2022 at 8:22 PM<br>
<b>To: </b>3DEM Mailing List <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>[3dem] Excessive drift/motion on 2nm ultrathin carbon coated grids</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Helvetica Neue"">Dear all,</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Helvetica Neue"">I am collecting data with 2nm ultrathin carbon coated Quantifoil holy grids and observing excessive/abnormal drift/motion in images which I did not see with regular Quantifoil
grids or other type of grids. There is no indication of the microscope as the cause of drift/motion. I am curious if anyone else has also observed the similar motion/drift with 2nm ultrathin carbon coated grids. FFTs w/ and w/o drift/motion are attached as
references. Any feedback insight will be appreciated.</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Helvetica Neue"">Thanks,</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Helvetica Neue"">Guobin</span><o:p></o:p></p>
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