[3dem] cryo-EM/cryo-ET access at CBI via iNEXT-Discovery

[Bruno KLAHOLZ]

Dear all,

this is to inform interested users from academics and industry that the Centre for Integrative Biology (CBI) / IGBMC at Illkirch/Strassburg, France is happy to offer - as part of the iNEXT-Discovery infrastructure - 3 types of access modalities:

- high-resolution single particle cryo-EM
- enhenced support for cryo-EM ("from sample to classes": which includes negative staining, cryo-EM tests & sample optimization tests on characterized protein samples, grid screening, early/mid stage cryo-EM data collection)
- cryo electron tomography

https://urldefense.proofpoint.com/v2/url?u=https-3A__inext-2Ddiscovery.eu_submit-2Dproposal&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=7eIZ0YjIWkLlPC5CNnyAMupXHUZt1JcHajtOMAQyxlY&e= 

Best regards,

Bruno Klaholz



Cryo-EM facilities at CBI/IGBMC:

The cryo-EM facility has Titan Krios and Glacios cryo electron microscopes for cryo-EM and tomography, cryo-FIB/SEM, super-resolution imaging (dSTORM), and a suite of associated equipments for sample preparation (grid freezing with Vitrobot and new Chameleon, HPF) and dedicated computing resources for image analysis, processing and 3D reconstruction by fluorescence microscopy, single particle cryo-EM (including on-the-fly data processing) and cryo electron tomography (cryo-ET). The Titan Krios microscope is equipped with Volta phase plate (VPP) and Cs corrector, GIF energy filter and K3 & Falcon 3 cameras, the Glacios with a K2 camera and a Ceta D camera for micro electron diffraction (μED); a further 300 kV instrument is to be installed 2022/2023.

The Centre for Integrative Biology (CBI) at IGBMC, Illkirch/Strasbourg, France, provides a cutting-edge scientific and technological environment in integrated structural biology to address the structure and function of biological systems, notably on gene expression, from the atomic, molecular to the tissue scales. The CBI https://urldefense.proofpoint.com/v2/url?u=http-3A__www.igbmc.fr_grandesstructures_cbi&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=f4_j5sBelJ8sc8lR5aSAas4x7m02JFXQXJUoBLvrSws&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.igbmc.fr_grandesstructures_cbi_&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=wH6QiNF3xopUSc4W1dmwY6W34Y02oTcW3V-jZrSZ21A&e= > hosts the French and European Infrastructures for Integrated Structural Biology, FRISBI https://urldefense.proofpoint.com/v2/url?u=https-3A__frisbi.eu&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=_LKR_P6LstyeJsLojTzhRsF5_05XygKVewmr-YSNrYk&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__frisbi.eu_&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=wsrVDJhtqGUNwWfIRJOawjDsjOxeCtQypni62zHbhJs&e= >, Instruct-ERIC https://urldefense.proofpoint.com/v2/url?u=https-3A__www.structuralbiology.eu&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=wGBODpDnrwLfwAqaAKl4DSSRkvZKuy0nhbmt_eE4Bxc&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.structuralbiology.eu_&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=tMzYCLoTATbsq0iA3ucJnYY0Qi2_sFyk_RMflqspheU&e= > and iNext-Discovery https://urldefense.proofpoint.com/v2/url?u=https-3A__inext-2Ddiscovery.eu&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=bXYJMb926c-xtx0JrCY2gQIj0_cPAdiP3XndCrwk4tk&e= . Nodes of FRISBI and Instruct-ERIC also provide access for protein sample production, characterisation and structural analysis.



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Bruno P. Klaholz
Centre for Integrative Biology
Department of Integrated Structural Biology
Institute of Genetics and of Molecular and Cellular Biology
IGBMC - UMR 7104 - U 1258
1, rue Laurent Fries
BP 10142
67404 ILLKIRCH CEDEX
FRANCE
Tel. from abroad: 0033.369.48.52.78
Tel. inside France: 03.69.48.52.78
websites:
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.igbmc.fr_Klaholz&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=Rumn7p75eIeLhF8kuxnl5G7aYKCxLcCKxoWZxoW9vos&e= 
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.igbmc.fr_grandesstructures_cbi&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=f4_j5sBelJ8sc8lR5aSAas4x7m02JFXQXJUoBLvrSws&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.igbmc.fr_grandesstructures_cbi_&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=wH6QiNF3xopUSc4W1dmwY6W34Y02oTcW3V-jZrSZ21A&e= >
https://urldefense.proofpoint.com/v2/url?u=https-3A__frisbi.eu&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=_LKR_P6LstyeJsLojTzhRsF5_05XygKVewmr-YSNrYk&e= 
https://urldefense.proofpoint.com/v2/url?u=http-3A__instruct-2Deric.com&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=5Qutn0KYgZdpVRok6y7YK7mFbdZ0msrDyKklvnv2UvM&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__instruct-2Deric.com_&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=ePQwP3ZpfujbEf3BBb9hz1v1qsmJvZkG6YpdriuEYLI&e= >
https://urldefense.proofpoint.com/v2/url?u=https-3A__inext-2Ddiscovery.eu&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=bXYJMb926c-xtx0JrCY2gQIj0_cPAdiP3XndCrwk4tk&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__inext-2Ddiscovery.eu_&d=DwIFCQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=TSHrovwmas0Sfg5d1sgK3xgJT8ORaZyS70uJy1kuPRhCvRhTdch-tX0O-obZ4JsX&s=1Zg5YwDUN9e-zYvl9Rx6c_0uWNiTywB2-j40KrubksE&e= >


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