[3dem] GMS3.5 shutdown experiences

Morgan, David Gene dagmorga at indiana.edu
Fri Apr 8 14:26:15 PDT 2022


We have been running 3.5 for a K3 attached to a Talos Arctica for many months now and have not ever experienced a shutdown like you describe.  We've definitely had other issues, but no unexplained shutdowns.

    politics is more difficult than physics.
                                             A. Einstein

            David Gene Morgan
        Electron Microscopy Center
             047E Simon Hall
             IU Bloomington
          812 856 1457 (office)
          812 856 3221 (3200)
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Marcus FISLAGE <Marcus.Fislage at vub.be>
Sent: Friday, April 8, 2022 3:49 AM
To: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: [External] [3dem] GMS3.5 shutdown experiences

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Dear all,

since we upgraded our Camera software from Gatan to version 3.5 (K3 camera on JEOL CryoARM300 using SerialEM 4.0.1-4) we experienced regular full shutdowns of the GMS software. I.e. no error message and the whole software package is just not running anymore. And a simple reopening of the GMS3 software works and I can continue the data collection.
I am already in contact with our Gatan engineer, but he is rather surprised about this behavior.

I would therefore like to ask the crowd if you have had or currently have a similar experience.

To the best of my knowledge, it appears that the frequency of GMS shutdown is correlated to the number of images taken. I.e. After a certain threshold of images taken with the K3 camera, the GMS software will just disappear.
I currently tested it by a daily (or every second day) restart of the GMS software and since then the error did not appear again for 2 weeks. This would initially support my theory.

Any feedback or comments would be highly appreciated.



Dr. Marcus Fislage

EM Manager

VIB-Center for Structural Biology
VUB (Vrije Universiteit Brussel)
Building P1T, room 0.01
Pleinlaan 2

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