[3dem] Uranyl acetate alternative

[Schwartz, Cindi (NIH/NIAID) [E]]

For section staining, I’ve used 2% aqueous samarium acetate (prepared essentially the same as 2% UA). Samarium acetate waste (at our institution) is just added to some absorbent pellets and then put through our in-house incinerator. I use 1% Samarium acetate in en bloc staining of TEM samples before embedding in resin. It seems to work just fine there too.

However, the stain is somewhat weak for sections and if I need something a bit stronger, I do use 2% UA. For en bloc staining, it seems to be about equal to UA.

I have never tried the SA for negative staining.

I hope that helps,
Cindi L. Schwartz
(she/her/hers)

Electron Microscopist
Rocky Mountain Labs/NIAID/NIH
903 South 4th Street
Hamilton, MT  59840
406-363-9228
Cindi.Schwartz at NIH.gov<mailto:Cindi.Schwartz at NIH.gov>

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From: Denis Chrétien <denis.chretien at univ-rennes1.fr>
Sent: Wednesday, October 20, 2021 1:36 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Uranyl acetate alternative

Dear all,

It becomes difficult to posses Uranyl Acetate salts. Hence we would like to have your feedback concerning alternatives for sections and negative staining. Along this line, we would appreciate your experience concerning this salt :

https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_SDS-5Fhtml_19485-5Fsds.pdf&d=DwIGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=cO6GRdtNJiTWnwDrJGYbsGv1wR8cU5kVGIEmwqG6f18&s=YB0HHKO6pERqN8_KrCD6ybLVXuC9iCsS_zUMlM4wCSg&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_SDS-5Fhtml_19485-5Fsds.pdf&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=hRDyTdJW4hH05tkZJrE0XkfrezWAxeD53hmQWoZC2pk&s=SzdIxK_0wo-0i8bTfRNyPQnZX2l3AyLDYJwGNuZsMTs&e=>

Other suggestions are welcomed!

Best,

Denis
___________________________
Université de Rennes 1
UMR 6290, équipe TIPs
Campus de Beaulieu, Bât. 13
35042 Rennes Cedex
France

Tel. +33 (0)2 23 23 67 64
https://urldefense.proofpoint.com/v2/url?u=https-3A__igdr.univ-2Drennes1.fr_&d=DwIGaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=cO6GRdtNJiTWnwDrJGYbsGv1wR8cU5kVGIEmwqG6f18&s=1xpLczdgk6XD1yuxdcosiWgcTF78kvia23YWP7VVkKI&e= <https://urldefense.proofpoint.com/v2/url?u=https-3A__igdr.univ-2Drennes1.fr_&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=hRDyTdJW4hH05tkZJrE0XkfrezWAxeD53hmQWoZC2pk&s=J9NRrJh9gl0QKmDtkcBNTdu6xKGp5rtgq6N8Gs-jWos&e=>
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