<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<!--[if !mso]><style>v\:* {behavior:url(#default#VML);}
o\:* {behavior:url(#default#VML);}
w\:* {behavior:url(#default#VML);}
.shape {behavior:url(#default#VML);}
</style><![endif]--><style><!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:"Lucida Handwriting";
panose-1:3 1 1 1 1 1 1 1 1 1;}
@font-face
{font-family:"Iowan Old Style Roman";}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
font-size:11.0pt;
font-family:"Calibri",sans-serif;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}
span.EmailStyle18
{mso-style-type:personal-reply;
font-family:"Calibri",sans-serif;
color:windowtext;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
</head>
<body lang="EN-US" link="blue" vlink="purple" style="word-wrap:break-word">
<div class="WordSection1">
<p class="MsoNormal">For section staining, I’ve used 2% aqueous samarium acetate (prepared essentially the same as 2% UA). Samarium acetate waste (at our institution) is just added to some absorbent pellets and then put through our in-house incinerator. I use
1% Samarium acetate in en bloc staining of TEM samples before embedding in resin. It seems to work just fine there too.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">However, the stain is somewhat weak for sections and if I need something a bit stronger, I do use 2% UA. For en bloc staining, it seems to be about equal to UA.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I have never tried the SA for negative staining.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I hope that helps,<o:p></o:p></p>
<div>
<p class="MsoNormal"><span style="font-family:"Lucida Handwriting";color:black">Cindi L. Schwartz</span><span style="font-family:"Arial",sans-serif;color:black"> </span><o:p></o:p></p>
<p class="MsoNormal">(she/her/hers)<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black">Electron Microscopist</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black">Rocky Mountain Labs/NIAID/NIH</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black">903 South 4th Street</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black">Hamilton, MT 59840</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black">406-363-9228</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black"><a href="mailto:Cindi.Schwartz@NIH.gov" target="_blank">Cindi.Schwartz@NIH.gov</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><img border="0" width="189" height="47" style="width:1.9687in;height:.4895in" id="Picture_x0020_1" src="cid:image001.jpg@01D7C589.C5F247A0" alt="Image of a check mark.
Text: "I got my COVID-19 vaccine.""><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="font-size:13.0pt;font-family:"Iowan Old Style Roman";color:black">***********************************************************************</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Iowan Old Style Roman";color:black">The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended
recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's
own and not expressly made on behalf of the NIAID by one of its representatives. </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:13.0pt;font-family:"Iowan Old Style Roman";color:black">***********************************************************************</span><o:p></o:p></p>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<div style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b>From:</b> Denis Chrétien <denis.chretien@univ-rennes1.fr>
<br>
<b>Sent:</b> Wednesday, October 20, 2021 1:36 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] Uranyl acetate alternative<o:p></o:p></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Dear all,<o:p></o:p></p>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">It becomes difficult to posses Uranyl Acetate salts. Hence we would like to have your feedback concerning alternatives for sections and negative staining. Along this line, we would appreciate your experience concerning this salt :<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.tedpella.com_SDS-5Fhtml_19485-5Fsds.pdf&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=hRDyTdJW4hH05tkZJrE0XkfrezWAxeD53hmQWoZC2pk&s=SzdIxK_0wo-0i8bTfRNyPQnZX2l3AyLDYJwGNuZsMTs&e=">https://www.tedpella.com/SDS_html/19485_sds.pdf</a><o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">Other suggestions are welcomed!<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">Best,<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">Denis<o:p></o:p></p>
</div>
<div>
<div>
<div>
<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="color:black">___________________________<br>
Université de Rennes 1<br>
UMR 6290, équipe TIPs<br>
Campus de Beaulieu, Bât. 13<br>
35042 Rennes Cedex<br>
France<br>
<br>
Tel. +33 (0)2 23 23 67 64<br>
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__igdr.univ-2Drennes1.fr_&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=hRDyTdJW4hH05tkZJrE0XkfrezWAxeD53hmQWoZC2pk&s=J9NRrJh9gl0QKmDtkcBNTdu6xKGp5rtgq6N8Gs-jWos&e=">https://igdr.univ-rennes1.fr/</a><br>
___________________________<br>
<br>
<br>
<br>
<o:p></o:p></span></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
</div>
</body>
</html>