[3dem] Membrane protein dissociating from nanodisc

[Pradeep HN]

Hi,

I assume that you are using one of the MSPs and lipid/lipid mixture! If
yes, is the molar ratio of the lipids you added is undersaturated?

Best,
Pradeep

On Fri, 15 Oct 2021 at 15:13, Dykstra, Holly <Holly.Dykstra at vai.org> wrote:

> Hello all,
>
> I am working on a single-TM membrane protein in nanodisc. Upon freezing
> grids, I am observing protein dissociation from the nanodisc. A small
> percentage of the total particle population is intact protein-nanodisc
> complex but most of the particles have dissociated. I believe this is
> happening at the point of freezing as negative stain shows the intact
> protein-nanodisc complex. Has anyone else experienced this problem or does
> anyone have any suggestions or advice?
>
> Thanks!
>
> -Holly
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Pradeep Hiriyur, | CEITEC MU

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