[3dem] [EXTERNAL] K3 problem during warming up

[Craig Yoshioka]

Yes we see both quite a bit, the errors messages during annealing are sporadic- we ignore them for the most part.  

The other error is just a good old fashioned DM crash.  Sometime you can narrow it down to a specific action that caused it, other times it remains a mystery.  One thing I can note is that DM often does not gracefully handle being told to do something from SerialEM while the camera is active in DM.  Another is that switching between CDS and non-CDS mode, especially from SerialEM, is a bit of a gamble, perhaps the camera re-initializtion is too variable to reliably wait for.  In the past I’ve also seen some issues related to the GPU frame alignment in the SerialEM DM plugin- but I have not seen this in very long while- then again we only enable frame alignment in SerialEM when we are screening or setting up data collection.






> On Oct 14, 2021, at 12:36 PM, shixin yang <shxyang2000 at yahoo.com> wrote:
> 
> Dear colleagues,
> 
> Our K3 camera in krios has two problems:
> 
> 1. When we carry out cryo cycle in the weekend, K3 camera will be warmed up. Two messages pop up during warming up K3 camera (see attached). The first message ("The sensor is off") will show up after ~ 20 minutes warming up. The temperature increase to 37 degree in K3 camera. Another message (the sensor is on)  will show up when temperature drops down in camera. The camera still works fine in spite of these two messages. Does anyone observe this message? Does this message imply any damage in the camera sensor?
> 
> 2. Another message occasionly pops up during data collection (see 2nd attachment) in SerialEM. It indicates that DM stops work. We can't click any other place in windows desktop except this message window. After clicking "close the program", DM will be automatically be shutdown. Camera can be restored to work after restart DM.
> 
> Any input will be greatly appreciated.
> 
> Thanks
> 
> Shixin
> <SensorOffK3Camera.jpg><DM_Stop_Working.JPG>_______________________________________________
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