[3dem] Upcoming Webinar November 16 | Cryo-tomography with Gaia Pigino

[Nylese, Tara]

Hello All,

I'd like to take a moment to invite you to join us for an upcoming webinar:

Towards a mechanistic understanding of motile and primary cilia with CLEM, cryo-electron tomography and expansion microscopy
Date: November 16, 2021
Time: 10:00am PST, 1:00pm EST, 7:00pm CET

Featuring: Gaia Pigino
Associate Head of Structural Biology Research Centre, Human Technopole

Register:
https://urldefense.proofpoint.com/v2/url?u=https-3A__www.labroots.com_webinar_towards-2Dmechanistic-2Dunderstanding-2Dmotile-2Dprimary-2Dcilia-2Dclem-2Dcryo-2Delectron-2Dtomography-2Dexpansion-2Dmicro&d=DwIFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=GDYqO_KGdhUcivVWg4GtnzcIj96ncLT5lhyZl4ksUl084P4CjCIrth7zELtweMxY&s=_hnYcLnbO63wcoUqy6N2rTBNAp21TOx0tpjG_sgoljY&e= 

Abstract:
In this talk I will show how the power of modern electron microscopy (EM) techniques, coupled with light microscopy and expansion methods, can open a window to the molecular dynamics of cells, enabling us to discover details about cellular processes at unprecedented level of detail. More concretely, I will show how we have used time resolved correlative light and electron microscopy (CLEM), cryo-FIB-SEM/cryo-electron tomography (cryo-ET) and expansion microscopy (U-ExM) paired with confocal fluorescence microscopy, TIRF microscopy and mechanical manipulations of cells to study a number of essential molecular processes in motile and primary cilia, i.e. conserved organelles that are fundamental for most eukaryotic cells. While our own work focuses on the inner workings of cilia, many open questions in cell and molecular biology can be addressed by combining modern imaging methods and methods from cell biology in suitable ways.

Learning Objectives:

  *   Discuss how cryo-electron tomography (cryo-ET) is used for imaging the interior of cells at high resolution
  *   Describe how cryo-ET can be combined with cell biology methods thru correlative light electron microscopy
  *   Discuss new understanding of the processes involved in assembly and function of motile and primary cilia, conserved organelles that are fundamental for most eukaryotic cells


Best Regards,
Tara

Tara Nylese
Marketing Development Manager, Americas
Life Sciences
Materials & Structural Analysis
Thermo Fisher Scientific
Tara.Nylese at ThermoFisher.com<mailto:Tara.Nylese at ThermoFisher.com>
(845)664-8445

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