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<p class="MsoNormal" style="line-height:15.0pt">Hello All,<o:p></o:p></p>
<p class="MsoNormal" style="line-height:15.0pt"><span style="font-size:10.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal" style="line-height:15.0pt">I’d like to take a moment to invite you to join us for an upcoming webinar:
<o:p></o:p></p>
<p class="MsoNormal" style="line-height:15.0pt"><o:p> </o:p></p>
<p class="MsoNormal" style="line-height:15.0pt"><b>Towards a mechanistic understanding of motile and primary cilia with CLEM, cryo-electron tomography and expansion microscopy<o:p></o:p></b></p>
<p class="MsoNormal" style="line-height:15.0pt">Date: November 16, 2021<b><o:p></o:p></b></p>
<p class="MsoNormal">Time: 10:00am PST, 1:00pm EST, 7:00pm CET<o:p></o:p></p>
<p class="MsoNormal" style="line-height:15.0pt"><o:p> </o:p></p>
<p class="MsoNormal">Featuring: Gaia Pigino<o:p></o:p></p>
<p class="MsoNormal">Associate Head of Structural Biology Research Centre, Human Technopole<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Register:<o:p></o:p></p>
<p class="MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.labroots.com_webinar_towards-2Dmechanistic-2Dunderstanding-2Dmotile-2Dprimary-2Dcilia-2Dclem-2Dcryo-2Delectron-2Dtomography-2Dexpansion-2Dmicro&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=GDYqO_KGdhUcivVWg4GtnzcIj96ncLT5lhyZl4ksUl084P4CjCIrth7zELtweMxY&s=_hnYcLnbO63wcoUqy6N2rTBNAp21TOx0tpjG_sgoljY&e=">https://www.labroots.com/webinar/towards-mechanistic-understanding-motile-primary-cilia-clem-cryo-electron-tomography-expansion-micro</a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Abstract: <o:p></o:p></p>
<p class="MsoNormal">In this talk I will show how the power of modern electron microscopy (EM) techniques, coupled with light microscopy and expansion methods, can open a window to the molecular dynamics of cells, enabling us to discover details about cellular
processes at unprecedented level of detail. More concretely, I will show how we have used time resolved correlative light and electron microscopy (CLEM), cryo-FIB-SEM/cryo-electron tomography (cryo-ET) and expansion microscopy (U-ExM) paired with confocal
fluorescence microscopy, TIRF microscopy and mechanical manipulations of cells to study a number of essential molecular processes in motile and primary cilia, i.e. conserved organelles that are fundamental for most eukaryotic cells. While our own work focuses
on the inner workings of cilia, many open questions in cell and molecular biology can be addressed by combining modern imaging methods and methods from cell biology in suitable ways.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Learning Objectives:<o:p></o:p></p>
<ul style="margin-top:0in" type="disc">
<li class="MsoListParagraph" style="margin-left:0in;mso-list:l0 level1 lfo1">Discuss how cryo-electron tomography (cryo-ET) is used for imaging the interior of cells at high resolution<o:p></o:p></li><li class="MsoListParagraph" style="margin-left:0in;mso-list:l0 level1 lfo1">Describe how cryo-ET can be combined with cell biology methods thru correlative light electron microscopy<o:p></o:p></li><li class="MsoListParagraph" style="margin-left:0in;mso-list:l0 level1 lfo1">Discuss new understanding of the processes involved in assembly and function of motile and primary cilia, conserved organelles that are fundamental for most eukaryotic cells<o:p></o:p></li></ul>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Best Regards,<o:p></o:p></p>
<p class="MsoNormal">Tara<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><b>Tara Nylese<o:p></o:p></b></p>
<p class="MsoNormal">Marketing Development Manager, Americas<o:p></o:p></p>
<p class="MsoNormal">Life Sciences<o:p></o:p></p>
<p class="MsoNormal">Materials & Structural Analysis<o:p></o:p></p>
<p class="MsoNormal">Thermo Fisher Scientific <o:p></o:p></p>
<p class="MsoNormal"><a href="mailto:Tara.Nylese@ThermoFisher.com">Tara.Nylese@ThermoFisher.com</a><o:p></o:p></p>
<p class="MsoNormal">(845)664-8445<o:p></o:p></p>
<p class="MsoNormal"><b><span style="color:red"><o:p> </o:p></span></b></p>
<p class="MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.ascb.org_cellbio2021_registration_-3Futm-5Fsource-3Dascb-2Dmeeting-2Dwebsite-26utm-5Fmedium-3Dtop-2Dcta-26utm-5Fcampaign-3D2021-2Dvirtual-2Dcell-2Dbio-2Dmeeting&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=GDYqO_KGdhUcivVWg4GtnzcIj96ncLT5lhyZl4ksUl084P4CjCIrth7zELtweMxY&s=bUFk-hWWtzLZpVPMvYfKQCYWvD0y5MeQwzwNJGutMkQ&e="><span style="color:windowtext;text-decoration:none"><img border="0" width="600" height="125" style="width:6.25in;height:1.3055in" id="Picture_x0020_1" src="cid:image001.jpg@01D7DA42.9FFBE060"></span></a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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