[3dem] Fwd: cryo TEM holder for autogrids and ThermoFisher/FEI

[Szymon W Manka]

Hi Garry,

The transfer station for the Simple Origin holder is excellent. It is
sturdy and light-weight, very light compared to the 626 transfer station,
so its easy to handle and the built in LEDs are great. It also has a built
in heater. Overall, very happy with it.

We use Model 200 for classic bare grids (not AutoGrids) and they are fairly
easy to load and clamp. I reckon AutoGrids would be even easier to load and
centre as they are heavier and don't dance so much.

The holder is stable. It is centro-symmetric, which is good for stability
at tilts and I believe the wand is not in direct contact with LN2 in the
Model 200 dewar, which helps to keep vibrations at bay. Comparable to 626
in my hands though, never had big issues with it either.

LN2 lasts long, feels like it keeps much better than in 626, but never
tested extensively.

All the best,
Szymon


On Mon, Jun 14, 2021 at 6:32 PM Garry P Morgan <Garry.Morgan at colorado.edu>
wrote:

> Tobias,
> we’ve been using the UH tilt Elsa holder over the last year…
> pro’s:
> -we get ~10-11 hours of stable imaging time for overnight acquisition
> -it’s much more stable than our 626 holders
>
> con’s:
> -we see a higher occcurrence of crystalline/non-vitreous ice; i believe
> this is at least partly due to the thin tip on the UH tilt version of the
> holder
> -more column vacuum crashes when inserting into our G2 series Tecnai TEMs;
> i’m pretty sure is due to the excessive weight of the holder and large
> dewar, especially when it’s on the full side with LN2.  i’ve actually come
> up with some tweaks to inserting the holder that have resulted in
> dramatically reducing the number of vacuum crashes…since this is a bit
> off-topic from the autogrid question, i won’t get into the details, but
> anyone interested in having fewer vacuum crashes please contact me.
>
> i’m curious to know more about the Model 200 holder after your 2 week
> trial, as we’ll be looking into this as well in the very near future…i saw
> the response from Syzmon last week, but curious to know more:
> -were you happy with the transfer station? is it comparable to the 626
> station?
> -is the process of loading auto-grids into this holder pretty straight
> forward and simple?
> -how long does the LN2 last?
> -how does the stability compare to a 626 holder?
>
> cheers,
> Garry
>
> ________________________________
> Garry Morgan
> Director  —  Boulder EM Services —  Department of MCD Biology
> Campus Box 347  —  University of Colorado  —  Boulder, CO 80309
> phone:  303-492-8402
>
>
> On Jun 8, 2021, at 11:51 PM, Tobias Furstenhaupt <furstenh at mpi-cbg.de>
> wrote:
>
> Dear colleagues,
>
> So far we have two 626 Gatan cryoholders but we now look for a new cryo
> holder for our side-entry Cryo TEM (Titan Halo) to image autogrids. To my
> knowledge there are two companies offering those holders in the new
> 'toroid' design with temperature holding time of about 10h:
>
> - Gatan with its "Elsa" holder for one or three autogrids
> - Simple Origins Model 200 for two autogrids
>
> does anybody of you know other sources? Do you have any experiences with
> the the Elsa or 200? We tested the latter for two weeks and it works as
> promised without problems.
>
> cheers
> Tobias
>
>
> -----------------------------------
> Tobias Fürstenhaupt, PhD
> head of Electron Microscopy
> Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
> Pfotenhauerstrasse 108
> 01307 Dresden, Germany
>
> mail: furstenh at mpi-cbg.de
> phone: (+49) (0)351 210-2690
> cell: (+49) (0)176 / 44498706 (NEW!)
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>
>
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20210615/97c3a30c/attachment.html>