[3dem] Cryo Storage

[Watt, John Daniel]

Dear Colleagues,


We are seeking good solutions for ice-free storage of vitrified samples. We use a Vitrobot and front loading TEMs only, so no autogrids. Currently we place the grids in a plastic sample puck, then into a 50 mL Falcon tube and store in a Worthington 35LDB LN2 dewar. We see some frosting of the polystyrene lid, but dry this periodically to minimize frost. Our lab is controlled to 40 % humidity, which unfortunately we cannot change.


Are there any good off-the-shelf solutions for ice-free storage that we are missing?


Many thanks,


John Watt, PhD

Center for Integrated Nanotechnologies

Los Alamos National Laboratory

Office: +1-505-284-0018

Cell: +1-505-206-1523

Email: watt at lanl.gov
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20210121/462c7276/attachment.html>