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<p>Dear Colleagues, <br>
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<p>We are seeking good solutions for ice-free storage of vitrified samples. We use a Vitrobot and front loading TEMs only, so no autogrids. Currently we place the grids in a plastic sample puck, then into a 50 mL Falcon tube and store in a Worthington 35LDB
LN2 dewar. We see some frosting of the polystyrene lid, but dry this periodically to minimize frost. Our lab is controlled to 40 % humidity, which unfortunately we cannot change.
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<p>Are there any good off-the-shelf solutions for ice-free storage that we are missing?</p>
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<p>Many thanks, <br>
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<p><span style="font-family:Constantia,"Hoefler Text",serif"><span style="color:rgb(0,111,201); font-size:10pt; font-family:Constantia,"Hoefler Text",serif">John Watt, PhD</span></span><br>
<span style="color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif; font-size:10pt"></span><span style="font-size:11pt"></span></p>
<span style="color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif; font-size:10pt"></span><span style="font-size:11pt"></span><span style="color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif; font-size:10pt"></span>
<p><font face="Constantia, Hoefler Text, serif" color="#006fc9"><span style="font-size:10pt; color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif">Center for Integrated Nanotechnologies</span></font></p>
<span style="color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif; font-size:10pt"></span><span style="font-size:11pt"></span><span style="color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif; font-size:10pt"></span>
<p><font face="Constantia, Hoefler Text, serif" color="#006fc9"><span style="font-size:10pt; color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif">Los Alamos National Laboratory</span></font></p>
<span style="color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif; font-size:10pt"></span><span style="font-size:11pt"></span><span style="color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif; font-size:10pt"></span>
<p><font face="Constantia, Hoefler Text, serif" color="#006fc9"><span style="font-size:10pt; color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif">Office: +1-505-284-0018</span></font></p>
<span style="color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif; font-size:10pt"></span><span style="font-size:11pt"></span><span style="color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif; font-size:10pt"></span>
<p><font face="Constantia, Hoefler Text, serif" color="#006fc9"><span style="font-size:10pt; color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif">Cell: +1-505-206-1523</span><br>
<span style="color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif; font-size:10pt"></span><span style="font-size:11pt"></span></font></p>
<span style="color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif; font-size:10pt"></span><span style="font-size:11pt"></span><span style="color:rgb(0,111,201); font-family:Constantia,"Hoefler Text",serif; font-size:10pt"></span>
<p><font face="Constantia, Hoefler Text, serif" color="#006fc9"><span style="font-size:11pt; color:rgb(0,0,0); font-family:Times,"Times New Roman",serif"><span style="font-size:10pt; font-family:Constantia,"Hoefler Text",serif; color:rgb(0,111,201)">Email:
</span><span style="font-size:10pt; font-family:Constantia,"Hoefler Text",serif; color:rgb(0,111,201)">watt@lanl.gov</span></span><br>
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