[3dem] Symmetry Matrix Differences Between Cryo-EM Software

[Rodarte, Justas V]

Hi all,

I’m a relative newcomer to the field of Cryo-EM, so my apologies if this question is easy/misplaced/previously answered. I’m working on a VLP with I symmetry that displays 80 spikes. Some programs (Relion, cisTEM) apply symmetry correctly when moving onto 3D ab-initio, while others (Cryosparc), don’t. I was able to trace this to the fact that these programs use different matrices to apply symmetry, an example is included below. Does anyone know why this is though? To me it appears to be an oversight that two popular programs apply the same symmetry differently?

Thank you all for any help or advice you can provide!

Best,
Justas Rodarte


I1 Sym Matrix Relion
rot_axis 2, 1 0 0

rot_axis 5, 0.85065080702670 0 -0.5257311142635
rot_axis 3, 0.9341723640 0.3568220765 0

I1 Sym Matrix Cryosparc
rot_axis 2, 0 1 0
rot_axis 5, -0.85065080702670 0 0.5257311142635
rot_axis 3, -0.9341723640 0.3568220765


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