[3dem] strange ice pattern in grid

[ABBY]

Hi Sylvain,


The buffer of my sample is PBS but there may be some formaldehyde and sucrose residue even after purification. 


Best,
Yutong Song




At 2021-12-18 21:44:04, "Trepout Sylvain" <Sylvain.Trepout at curie.fr> wrote:

Hi Yutong,

I have this from time to time.

That's definitely a contamination which occured during plunge-freezing or during clipping of the grid on the holder or during introduction to the microscope.

I would suspect some cooling issues or deposition of non-vitreous ice after plunge-freezing. I don't know your setup. Make sure you hve enough nitrogen evaporation during plunge-freezing. Make sure the clipping of the grid on the holdee is tight enough.

Another possibility: are your samples in pure water or are you using organic solvents (such as DMF, THF for instance).

Best wishes,
Sylvain
----------
Sylvain TREPOUT, PhD, HDR
IR2 Inserm
Plateforme de Microscopie Electronique
INSERM US43 / CNRS UMS2016
Institut Curie Centre De Recherche
Centre Universitaire, Bât. 101B-110-111-112
Rue Henri Becquerel
CS 90030
91401 ORSAY Cedex, FRANCE
Phone : +33 1 69 86 30 81
Fax : +33 1 69 07 53 27
De : 3dem <3dem-bounces at ncmir.ucsd.edu> de la part de ABBY <aaabey at 126.com>
Envoyé : samedi 18 décembre 2021 14:30:37
À : 3dem at ncmir.ucsd.edu
Objet : [3dem] strange ice pattern in grid
 
Hi everyone,


We have seen some strange ice in our micrographs from K3 like attachments. One is grain shape ice and another is wave shape ice, some area contain them both. We tried to get tilt series and made tomograms, finding the strange pattern only exist in the bottom but not go through ice layer. Have anyone seen this before and know how these strange ice form? I think there is no problem in our grids recycle and storage.


Thanks a lot!


Best regards,
Yutong Song


 




 
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