[3dem] strange ice pattern in grid

[Stahlberg Henning]

Hi,
I’d think your wave is Leopard skin contamination, where you sample in the EM is not cold enough. Water sublimates and recrystallizes nearby.
The other type could be residues from the grids. Did you try changing to another grid type?


Henning

On 18 Dec 2021, at 14:31, ABBY <aaabey at 126.com> wrote:


Hi everyone,

We have seen some strange ice in our micrographs from K3 like attachments. One is grain shape ice and another is wave shape ice, some area contain them both. We tried to get tilt series and made tomograms, finding the strange pattern only exist in the bottom but not go through ice layer. Have anyone seen this before and know how these strange ice form? I think there is no problem in our grids recycle and storage.

Thanks a lot!

Best regards,
Yutong Song







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