[3dem] Leica EM GP2 best practices

[Andrei Moiseenko]

Dear all,

I am trying to get reproducible results with Leica EM GP2. I would be very
grateful if you could share any successful best practices on using this
plunge freezer. 

Specifically:

1.       Do you use the blotting sensor? In my case it fails very often and
it seems that the results are better without it. 

2.       In case you use the blotting sensor, what range of "additional
movement" do you use?

3.       In case you use manual blotting, how do you set the horizontal blot
position: just by touching the grid with the filter paper, or slightly
bending it?

4.       How do you set the filter paper vertical position: covering the
grid only, just touching the forceps tips or higher (how much higher)? It
seems that setting the higher position helps to reduce the effects of the
filter paper crinkling.

5.       What range of blotting time and specimen volume do you typically
use for SPA samples at conditions specified?

Any advice on using the device is very welcome.

 

Best regards,
Andrey Moiseenko

Electron Microscopy Lab @ Biology Department
Moscow State University
 <mailto:postmoiseenko at gmail.com> postmoiseenko at gmail.com 

 

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