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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal>Dear all,<o:p></o:p></p><p class=MsoNormal>I am trying to get reproducible results with Leica EM GP2. I would be very grateful if you could share any successful best practices on using this plunge freezer. <o:p></o:p></p><p class=MsoNormal>Specifically:<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>Do you use the blotting sensor? In my case it fails very often and it seems that the results are better without it. <o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>2.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>In case you use the blotting sensor, what range of “additional movement” do you use?<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>3.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>In case you use manual blotting, how do you set the horizontal blot position: just by touching the grid with the filter paper, or slightly bending it?<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>4.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>How do you set the filter paper vertical position: covering the grid only, just touching the forceps tips or higher (how much higher)? It seems that setting the higher position helps to reduce the effects of the filter paper crinkling.<o:p></o:p></p><p class=MsoListParagraph style='text-indent:-18.0pt;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='mso-list:Ignore'>5.<span style='font:7.0pt "Times New Roman"'> </span></span><![endif]>What range of blotting time and specimen volume do you typically use for SPA samples at conditions specified?<o:p></o:p></p><p class=MsoNormal>Any advice on using the device is very welcome.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal style='margin-bottom:10.0pt;line-height:115%'>Best regards,<br>Andrey Moiseenko<o:p></o:p></p><p class=MsoNormal style='margin-bottom:10.0pt;line-height:115%'>Electron Microscopy Lab @ Biology Department<br>Moscow State University<br><a href="mailto:postmoiseenko@gmail.com"><span style='color:blue'>postmoiseenko@gmail.com</span></a> <o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>