[3dem] Using Thermo-Fisher Talos Arctica for room-temperature plastic-section tomography -things to consider

[Sonja Welsch]

Hello Pasi,

these are some things I still remember from my Eindhoven days and that 
you might want to consider (all are my personal opinion):


1. throughput: if the Autoloader (AL) is warm, cassette docking/sample 
loading will take longer than in cold state (as it takes longer for AL 
vacuum to recover). This will worsen with increasing number of docking 
rounds. Sample load = coffee break at some point.

2. If your customer wants to image multiple samples in one day (i.e. 
many sample exchanges) you might want to /consider asking your Service 
Engineer//to/ temporarily change/loosen the AL (PP AL) vacuum trip 
values to speed things up (trade-off of vacuum quality and speed).

3. You'll need to schedule a decent round of Autoloader and Column 
conditioning after a room temp session (especially if you have decided 
to go for 2.). That adds to the time needed for this session.

4. I think it's the (mechanically) safe option to operate Autoloader and 
column both at room temp - I'd never dare to operate column warm & AL 
cold.� I'm not even sure if all Talos SW versions allow sample loading 
with "one cold one warm"??

5. With conditioning column and Autoloader after a room temp session, 
there is no reason to assume the column is "dirty" or "contaminated" by 
any means.


Best wishes,

Sonja


On 12.04.21 14:06, Laurinm�ki, Pasi A wrote:
> Dear 3DEM community,
>
> We are operating a Talos Arctica that has been a good workhorse for 
> single-particle cryo-EM and cryo tomography.
> We now have a potential customer with plenty of experience in doing 
> room-temperature tomography from plastic sections.
>
> Thus, in short, we would be very interested in your experiences with 
> using Talos Arctica in room temperature for plastic-section tomography.
> Threats and issues? things to consider?
>
> BR,
> Pasi
>
> <<<<<<<<<<<<<<<<<<<>>>>>>>>>>>>>>>>>>
>
> Pasi Laurinm�ki�� PhD (biotech.)
>
> Laboratory Engineer & Cryo EM specialist
>
>
> Cryo-EM service
>
> Institute of Biotechnology
>
> University of Helsinki, Finland
>
> <<<<<<<<<<<<<<<<<<<>>>>>>>>>>>>>>>>>>
>
>
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