[3dem] using MTF during framealignemnt

[Jay Rai]

Actually I want to ask something like this..
Relion3.1 uses the MTF of the detector during refinement stages of refinement. So that which MTF of the detector should give the better results -CDS or standard ? Anyone has any idea!


With kind regards,

Jay Rai​

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Jay Rai <jrai at fsu.edu>
Sent: Tuesday, September 8, 2020 4:16 PM
To: Takanori Nakane <tnakane at mrc-lmb.cam.ac.uk>; 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] using MTF during framealignemnt

Thank you for the answer. The question was wrong. I was thinking of something else. But I did understand what you mean?


With kind regards,

Jay Rai​

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Takanori Nakane <tnakane at mrc-lmb.cam.ac.uk>
Sent: Tuesday, September 8, 2020 3:46 PM
To: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] using MTF during framealignemnt

Hi,

What do you mean by framealignment? Motion correction or Bayesian Polishing?

Both don't use an MTF file.

Best regards,

Takanori Nakane

On 2020/09/08 20:31, Jay Rai wrote:
> Hi EM community,
> Has anyone ever use MTF.star file during framealignement in relion 3.1? If so then which MTF is better, CDS mode or standard mode (K3 camera)?
> I am talking about the frame alignment of normal counting data.
> Any comments or suggestion are highly welcome.
>
> With kind regards,
>
> Jay Rai​
>
>
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