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Actually I want to ask something like this..</div>
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Relion3.1 uses the MTF of the detector during refinement stages of refinement. So that which MTF of the detector should give the better results -CDS or standard ? Anyone has any idea!</div>
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<span style="color:rgb(0,0,0); font-family:"Times New Roman",Times,serif; font-size:14pt">With kind regards,</span><br>
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<span style="font-family:"Times New Roman",Times,serif; font-size:14pt"></span><span style="font-family:inherit; font-size:inherit; font-style:inherit; font-variant-ligatures:inherit; font-variant-caps:inherit"><span style="font-size:14pt; color:rgb(0,0,0); font-family:"Times New Roman",Times,serif">Jay
Ra</span><span style="font-size:14pt; color:rgb(0,0,0); font-family:"Times New Roman",Times,serif">i</span><span style="font-size:14pt; color:rgb(0,0,0); font-family:"Times New Roman",Times,serif"></span></span></p>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Jay Rai <jrai@fsu.edu><br>
<b>Sent:</b> Tuesday, September 8, 2020 4:16 PM<br>
<b>To:</b> Takanori Nakane <tnakane@mrc-lmb.cam.ac.uk>; 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> Re: [3dem] using MTF during framealignemnt</font>
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Thank you for the answer. The question was wrong. I was thinking of something else. But I did understand what you mean?</div>
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<span style="color:rgb(0,0,0); font-family:"Times New Roman",Times,serif; font-size:14pt">With kind regards,</span><br>
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<span style="font-family:"Times New Roman",Times,serif; font-size:14pt"></span><span style="font-family:inherit; font-size:inherit; font-style:inherit; font-variant-ligatures:inherit; font-variant-caps:inherit"><span style="font-size:14pt; color:rgb(0,0,0); font-family:"Times New Roman",Times,serif">Jay
Ra</span><span style="font-size:14pt; color:rgb(0,0,0); font-family:"Times New Roman",Times,serif">i</span><span style="font-size:14pt; color:rgb(0,0,0); font-family:"Times New Roman",Times,serif"></span></span></p>
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<div id="x_divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" color="#000000" style="font-size:11pt"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Takanori Nakane <tnakane@mrc-lmb.cam.ac.uk><br>
<b>Sent:</b> Tuesday, September 8, 2020 3:46 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> Re: [3dem] using MTF during framealignemnt</font>
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<div class="x_PlainText">Hi,<br>
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What do you mean by framealignment? Motion correction or Bayesian Polishing?<br>
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Both don't use an MTF file.<br>
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Best regards,<br>
<br>
Takanori Nakane<br>
<br>
On 2020/09/08 20:31, Jay Rai wrote:<br>
> Hi EM community,<br>
> Has anyone ever use MTF.star file during framealignement in relion 3.1? If so then which MTF is better, CDS mode or standard mode (K3 camera)?<br>
> I am talking about the frame alignment of normal counting data.<br>
> Any comments or suggestion are highly welcome.<br>
> <br>
> With kind regards,<br>
> <br>
> Jay Rai<br>
> <br>
> <br>
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