[3dem] using MTF during framealignemnt

Takanori Nakane tnakane at mrc-lmb.cam.ac.uk
Tue Sep 8 12:46:49 PDT 2020


Hi,

What do you mean by framealignment? Motion correction or Bayesian Polishing?

Both don't use an MTF file.

Best regards,

Takanori Nakane

On 2020/09/08 20:31, Jay Rai wrote:
> Hi EM community,
> Has anyone ever use MTF.star file during framealignement in relion 3.1? If so then which MTF is better, CDS mode or standard mode (K3 camera)?
> I am talking about the frame alignment of normal counting data.
> Any comments or suggestion are highly welcome.
> 
> With kind regards,
> 
> Jay Rai​
> 
> 
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