[3dem] Spots in micrographs

[Bajaj Saumya (Dr)]

Dear all,

We are trying to solve the structure of a membrane protein. We have recently started seeing black spots (~1nm) on our micrographs (image attached). The pattern is not uniform, as there are areas where there are no spots. We tried changing buffer and detergent stock - that did not help. The grids are commercial graphene oxide girds and our buffer contains 0.03% GDN detergent.

Have any of you noticed something similar, or can help throw some light on what it could be?

Thanks a lot.
Best regards,
Saumya
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