[3dem] magnification question

[Michael Elbaum]

Just a heads-up then... I was shocked to see that the magnification written into a file name by the Talos software depends on the size of the monitor attached. Maybe this is STEM-specific? It's very misleading. Altogether magnification is a slippery concept though. The pixel sampling is much less ambiguous: what length in the specimen does a pixel in the recorded file represent? Multiply by the number of pixels for the field of view. It's the same image whether you view on your phone or projected onto the wall.


Michael

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Zongli Li <zongli.li at gmail.com>
Sent: Tuesday, June 23, 2020 11:38:26 PM
To: Morgan, David Gene
Cc: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] magnification question

Hi David Gene,

The magnifications reported by a Talos Arctica user interface should be " mag on the viewing screen" if the viewing screen is inserted, and will change to " mag on the CCD" when viewing screen is extracted. But as Sacha pointed out, what matters is really the calibrated mag on the whatever camera you have used for calibration and data collection, and this mag will not show up in the user interface, but stored in the magnification table in Gatan GMS if you are using Gatan CCD/K2/K3 camera.

Best,

Zongli

On Tue, Jun 23, 2020 at 1:50 PM Morgan, David Gene <dagmorga at indiana.edu<mailto:dagmorga at indiana.edu>> wrote:

Hi,


    Does anyone happen to know whether the magnifications reported by a Talos Artica are supposed to be "mag on the viewing screen" or "mag at the film plane"?  We clearly have a serious issue with the calibrated magnifications on our microscope, but I just recalibrated it and got more or less the same lousy magnifications.  I'm trying to understand our issues, and knowing what the microscope thinks it is reporting would be helpful.  Thanks in advance.


--
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            David Gene Morgan
        Electron Microscopy Center
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             IU Bloomington
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