[3dem] hydrogen plasmas for graphene UltrAuFoils

[Matthias Wolf]

Hi Tommy,

For graphene on UltrAuFoil, we are following Chris Russo's paper - also using 100% hydrogen plasma on Gatan Solarus. However, we like graphene oxide even better, because it is hydrophilic enough so we don't need to use the plasma cleaner, and we get larger patches. It is overall easier to use yet with similar results.

   Matthias

From: 3dem On Behalf Of White, Tommi A.
Sent: Tuesday, June 23, 2020 5:58 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] hydrogen plasmas for graphene UltrAuFoils

Hello 3DEM Community.

We are exploring Graphenea grids (UltrAuFoils with graphene coating) and exposure to hydrogen plasmas.  For specimens that were working fine with amorphous carbon films (lacey with carbon over the holes), we seem to get much less sample on Graphenea grids after normal glow discharge (PelcoEasiglow - 45sec, 15mAmp, 0.39mBarr).  We explored working with these same grids using 100% Hydrogen plasma (30 sccm, 100% power, 4e-4 Torr generated with Fischione 1070) for 10, 20 and 40 seconds.  Unfortunately with our specimen, we don't see an increase in protein adsorption as previously reported (https://urldefense.com/v3/__https://pubmed.ncbi.nlm.nih.gov/24747813/__;!!Mih3wA!VlvO5H57NHk53zdzYftIgo2klvTiPZPCoGsLGk-A3qK_KX9BiNEisCQt3Cq_SWAcSg$ <https://urldefense.com/v3/__https:/pubmed.ncbi.nlm.nih.gov/24747813/__;!!Mih3wA!W2ZEvZsHaXOh6L3SLsRIkcAObX7QU78SQu2aDuyHKfGaqJYmCxM1Ug1O9XzNql1J8w$>).  We have the grids held in a Pelco Grid clamping block for plasma treatment.  Can anyone provide tips/tricks?  Any other pretreatments needed?  We are open to all suggestions as the cost of these grids is a concern.

Thanks in advance,
Tommi A. White, Ph.D.
Director, Electron Microscopy Core
Assistant Research Professor, Biochemistry
University of Missouri
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