[3dem] Artica/Krios question

Thu Jun 18 00:19:40 PDT 2020

Hi David, 

We had a similar problem on our Arctica in the first year. We had this problem on version 1.9, if you have this version, ask an update to thermofisher to fix it. After that update, the problem was solved for us. 

Moreover, on this problem with the falcon we had two problems with the optic board. If EPU shows us a message : « Error while acquiring CDD image », we had to restart the CMTS to restart the Falcon3. 

If the protector show us « ? », we had to « stop all » in Microscope Software Launcher and « Start server and applications » . 

I hope this can help you, 

Best 

Armel 

Armel BEZAULT 
Engineer in cryo electron microscopy 
IECB - UMS3033/US001 
Université de Bordeaux, CNRS, Inserm 

[ mailto:a.bezault at iecb.u-bordeaux.fr | a.bezault at iecb.u-bordeaux.fr ] 
Phone. +33 (5) 4000 69 97 
Institut européen de chimie et biologie 
2, rue Robert Escarpit 
33607 Pessac, France 
[ https://urldefense.com/v3/__http://www.iecb.u-bordeaux.fr/index.php/fr/biologie-structurale/microscopie-electronique__;!!Mih3wA!Vb_H4ZPZxfAMDek2905ovQRLi0uDGAJS9WWGniWwN3RbTPEpE68nXYUVQUHa0pxP1w$  | https://urldefense.com/v3/__http://www.iecb.u-bordeaux.fr__;!!Mih3wA!Vb_H4ZPZxfAMDek2905ovQRLi0uDGAJS9WWGniWwN3RbTPEpE68nXYUVQUHszwTJdQ$  ] 

De: "Marta Carroni" <marta.carroni at scilifelab.se> 
À: "Mark Ebrahim" <mebrahim at mail.rockefeller.edu> 
Cc: 3dem at ncmir.ucsd.edu 
Envoyé: Jeudi 18 Juin 2020 08:54:42 
Objet: Re: [3dem] Artica/Krios question 

Hi David 
We had exactly the same here in Stockholm and Mark advised us to get the boards changed, which solved the problem. 
Good luck! 

Cheers, Marta 

Marta Carroni, SciLifeLab 
CryoEM Sweden 
Tomtebodavägen 23 A, gamma2 
17165, Solna 
Sweden 
Tel +468161013 
Tel +46720181462 

On 18 Jun 2020, at 00:06, Mark Ebrahim < [ mailto:mebrahim at mail.rockefeller.edu | mebrahim at mail.rockefeller.edu ] > wrote: 

Hi David, 

This is a problem, that most, if not all Talos systems face at some point. Some systems are more severe than others. While it is true that you would get the same error if your chiller were to "blip", that is likely not happening and there is probably nothing wrong with your chiller. 

The issue comes from some design flaw in the Talos optics boards which were a complete revamp from the Tecnai systems. On our Arctica at Rockefeller we had the same exact issue and it would manifest in the middle of the night during automated data collection- quite annoying. 

We went through 3-4 different optics boards until the problem went away. 

I believe FEI went through several iterations of "patches" to fix this issue. Since our issue was fixed by getting the right hardware I never installed any of these patches and I refuse to upgrade our software since we're in a state where everything works. I have not had the problem for several years. I can't say with certainty what the official FEI solution is. They may have a patch that works which could be installed by simply updating your software to the latest version (seemingly simple enough), or perhaps all the boards being manufactured now are free of this defect. 

You should share this information with your service engineer and ask him to open a TRF so he can get support from the next tier of engineers or from the factory in Holland. FEI knows about this issue and there should be a solution. 

Hope this was helpful. 

Best, 
Mark 

Mark Ebrahim 
Senior Staff Scientist 
Cryo Electron Microscopy Resource Center (CEMRC) 
The Rockefeller University 
1230 York Avenue 
New York, NY 10065 

From: 3dem < [ mailto:3dem-bounces at ncmir.ucsd.edu | 3dem-bounces at ncmir.ucsd.edu ] > on behalf of Morgan, David Gene < [ mailto:dagmorga at indiana.edu | dagmorga at indiana.edu ] > 
Sent: Wednesday, June 17, 2020 5:49 PM 
To: [ mailto:3dem at ncmir.ucsd.edu | 3dem at ncmir.ucsd.edu ] < [ mailto:3dem at ncmir.ucsd.edu | 3dem at ncmir.ucsd.edu ] > 
Subject: [3dem] Artica/Krios question 
Hi, 

We have a chronic problem with our Talos Artica sending out "optics board degraded" error messages. There is an easy reset for this, except that at least for our system, the Falcon camera can't be used after resetting the optics board unless one also does a microscope server reboot. Which my engineer says means I need to shut down and restart the FEG. So it's not an easy fix. 

This seems to happen once every few weeks, maybe once per month. Except that yesterday, it happened three times within about two hours. [I reset the optics but didn't use the Falcon, for those of you wondering how I could restart the FEG that quickly... ] It didn't happen today at all, so the error really seems to be happening randomly. 

My engineer says that the most likely cause of this is interruptions to the chilled water supply, but I have not been able to identify any sort of problem with flow, chilling, etc. Has anyone else seen similar chronic issues with this error? 

Thanks for any advice you can offer. 

-- 
politics is more difficult than physics. 
A. Einstein 

David Gene Morgan 
Electron Microscopy Center 
047E Simon Hall 
IU Bloomington 
812 856 1457 (office) 
812 856 3221 (3200) 
[ https://urldefense.com/v3/__http://iubemcenter.indiana.edu__;!!Mih3wA!XrwbBO8fwN7hgB7R39bs7d9iMdxh-CqMpju9Iy9RlzbNtCyfspTVrODU2TJBjx6Z0g$ | https://urldefense.com/v3/__http://iubemcenter.indiana.edu__;!!Mih3wA!Vb_H4ZPZxfAMDek2905ovQRLi0uDGAJS9WWGniWwN3RbTPEpE68nXYUVQUF9ceLM_g$  ] 
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