[3dem] Artica/Krios question

Alpay Burak Seven alburse at gmail.com
Wed Jun 17 22:32:47 PDT 2020


Server reboot shuts off HT but keeps FEG on in my experience too.

Alpay

On Wed, Jun 17, 2020, 6:33 PM Mike Strauss <mike.strauss at mcgill.ca> wrote:

> Hi David,
>
> when we had this problem (assuming the issue is the falcon protector) I
> seem to recall that a quick fix for the falcon was to change to increase
> the gun lens value by one click and then decrease it again.  If it is a
> communication issue with the falcon, then it’s probably a different story.
>
> The issue was indeed ultimately fixed by a software patch, after
> considerable trial and error.
>
> Regards,
> mike
>
> --------
> Mike Strauss
> Dep’t of Anatomy and Cell Biology
> McGill University
> 3640 University St.
> Strathcona Anatomy Bldg, Rm 144
> Montreal, QC H3A 0C7
> (514) 398-8084
> mike.strauss at mcgill.ca
>
> > On 17. Jun 2020, at 18:06, Mark Ebrahim <mebrahim at mail.rockefeller.edu>
> wrote:
> >
> > Hi David,
> >
> > This is a problem, that most, if not all Talos systems face at some
> point. Some systems are more severe than others. While it is true that you
> would get the same error if your chiller were to "blip", that is likely not
> happening and there is probably nothing wrong with your chiller.
> >
> > The issue comes from some design flaw in the Talos optics boards which
> were a complete revamp from the Tecnai systems. On our Arctica at
> Rockefeller we had the same exact issue and it would manifest in the middle
> of the night during automated data collection- quite annoying.
> >
> > We went through 3-4 different optics boards until the problem went away.
> >
> > I believe FEI went through several iterations of "patches" to fix this
> issue. Since our issue was fixed by getting the right hardware I never
> installed any of these patches and I refuse to upgrade our software since
> we're in a state where everything works. I have not had the problem for
> several years. I can't say with certainty what the official FEI solution
> is. They may have a patch that works which could be installed by simply
> updating your software to the latest version (seemingly simple enough), or
> perhaps all the boards being manufactured now are free of this defect.
> >
> > You should share this information with your service engineer and ask him
> to open a TRF so he can get support from the next tier of engineers or from
> the factory in Holland. FEI knows about this issue and there should be a
> solution.
> >
> > Hope this was helpful.
> >
> > Best,
> > Mark
> >
> > Mark Ebrahim
> > Senior Staff Scientist
> > Cryo Electron Microscopy Resource Center (CEMRC)
> > The Rockefeller University
> > 1230 York Avenue
> > New York, NY 10065
> > From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Morgan, David
> Gene <dagmorga at indiana.edu>
> > Sent: Wednesday, June 17, 2020 5:49 PM
> > To: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
> > Subject: [3dem] Artica/Krios question
> >
> > Hi,
> >
> >     We have a chronic problem with our Talos Artica sending out "optics
> board degraded" error messages.  There is an easy reset for this, except
> that at least for our system, the Falcon camera can't be used after
> resetting the optics board unless one also does a microscope server
> reboot.  Which my engineer says means I need to shut down and restart the
> FEG.  So it's not an easy fix.
> >
> >     This seems to happen once every few weeks, maybe once per month.
> Except that yesterday, it happened three times within about two hours.  [I
> reset the optics but didn't use the Falcon, for those of you wondering how
> I could restart the FEG that quickly... ]  It didn't happen today at all,
> so the error really seems to be happening randomly.
> >
> >     My engineer says that the most likely cause of this is interruptions
> to the chilled water supply, but I have not been able to identify any sort
> of problem with flow, chilling, etc.  Has anyone else seen similar chronic
> issues with this error?
> >
> >     Thanks for any advice you can offer.
> >
> > --
> >     politics is more difficult than physics.
> >                                              A. Einstein
> >
> >             David Gene Morgan
> >         Electron Microscopy Center
> >              047E Simon Hall
> >              IU Bloomington
> >           812 856 1457 (office)
> >           812 856 3221 (3200)
> >
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