[3dem] Tecnai T12 Camera config

[Mike Strauss]

Hi Krishna,

My guess is you will not be able to upgrade the microscope operating system. Tecnai instruments are sadly destined to die with windows xp.  In most cases, that means limited file sizes, limited RAM, and limited networking possibilities.   This also means Ceta cameras (by TFS) are not an option - since I am told they require Win7 at least.

If you’re upgrading your camera, make sure it comes with its own PC, which you can make modern, fancy and useful. TVIPS XF416 or Gatan OneView cameras would both give you great images, quick response, and at reasonable prices (compared with Direct Detectors). Both of these systems can be used with SerialEM (and probably Leginon, though I don’t know much about it) so you can use your own scripts, write your own plugins, and extract the microscope information you want to keep track of. SerialEM has a plugin to do frame alignment as you collect, so there’s no need to store frames. It also means you’d be doing the image processing, networking, data storage, and sending instructions to the microscope (via microscope PC) from a computer with better specs than the microscope PC, so it’s less likely to freeze.

Good luck.

Mike Strauss

--------
Prof. Mike Strauss
Anatomy and Cell Biology / FEMR
McGill University
(514) 398-8084
mike.strauss at mcgill.ca

> On 30. Jul 2020, at 14:37, Natesh Ramanathan <natesh at iisertvm.ac.in> wrote:
> 
> Dear Krishna,
> T12 with 4k x 4k will definitely work.  Computer is cheap now, so you can get it along with 4k x 4k upgrade.
> Perhaps others here will be able to enlighten you about Leginon and other requirements that you have.
> Best wishes,
> Natesh
> 
> On Thu, 30 Jul 2020 at 23:53, KrishnaM <vattipalli at gmail.com> wrote:
> Dear 3dem team,
> 
> We have a FEI Tecnai T12 Cryo-TEM with a eagle 2k × 2k camera. We are looking to upgrade the bottom mounted camera for it. 
> 
> Final goal is to acquire minimum of 4k × 4k images. Also required are automation in acquisition (like leginon), drift correction, ability to annotate, and having Audit trial traceability will be ultimate!
> 
> Our existing PC config is Windows XP (Stone aged!!). Wondering if this will still work with the upgradation of the camera. Any thoughts / suggestions will be appreciated.
> 
> Best Regards
> Krishna
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