[3dem] Filter paper cutter/puncher for Vitrobot

[Chambers, Melissa G]

We have Subangstrom’s punch.  You do have to start with 55mm round filter paper and this punches the hole in the center.

https://subangstrom.com/Accessories/Hole-Punch55mm-20mmID

In the past, we had an on campus machine shop make one but it wasn’t sharp enough to cut cleanly or easily.


Melissa

-----
Melissa G. Chambers

Director, Vanderbilt Cryo-EM Facility
Center for Structural Biology

Vanderbilt University
RR1207 MCN
1161 21st Ave S
Nashville TN 37232

615-322-4671
www.vanderbilt.edu/csb/facilities/cryo-electron-microscopy


From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Paolo Swuec <paolo.swuec at gmail.com>
Date: Monday, February 10, 2020 at 9:53 AM
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] Filter paper cutter/puncher for Vitrobot

Dear list,

We've been using commercially available filter paper for our Vitrobot. Given the cost of this consumable (5-10x the cost of Whatman filter paper 595), I am wondering whether anyone can recommend a paper cutter/puncher that can make some from filter paper sheet. I seem to remember the Gatan CP3 comes with one (if I'm not wrong, long time no see)...

Any advice?

Best,

Paolo

------------------------------------------------
Paolo Swuec, PhD
Cryo-Electron Microscopy Lab ❆ Facility Manager
Dep. BioSciences
University of Milano
Via Celoria 26 - 20133 (Italy)
Tel. +39 02 503 147 78

Follow on twitter @PS_CryoEM

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