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<p class="MsoNormal">We have Subangstrom’s punch. You do have to start with 55mm round filter paper and this punches the hole in the center.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><a href="https://subangstrom.com/Accessories/Hole-Punch55mm-20mmID">https://subangstrom.com/Accessories/Hole-Punch55mm-20mmID</a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">In the past, we had an on campus machine shop make one but it wasn’t sharp enough to cut cleanly or easily.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Melissa<o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;color:#3B3838">-----</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:#3B3838">Melissa G. Chambers</span><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:#3B3838"> </span><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:#3B3838">Director, Vanderbilt Cryo-EM Facility</span><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:#3B3838">Center for Structural Biology<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:#3B3838"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:#3B3838">Vanderbilt University<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:#3B3838">RR1207 MCN<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:#3B3838">1161 21<sup>st</sup> Ave S<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:#3B3838">Nashville TN 37232<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:#3B3838"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Verdana",sans-serif;color:#3B3838">615-322-4671<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Verdana",sans-serif">www.vanderbilt.edu/csb/facilities/cryo-electron-microscopy<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Paolo Swuec <paolo.swuec@gmail.com><br>
<b>Date: </b>Monday, February 10, 2020 at 9:53 AM<br>
<b>To: </b>"3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>[3dem] Filter paper cutter/puncher for Vitrobot<o:p></o:p></span></p>
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<p class="MsoNormal">Dear list, <o:p></o:p></p>
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<p class="MsoNormal">We've been using commercially available filter paper for our Vitrobot. Given the cost of this consumable (5-10x the cost of Whatman filter paper 595), I am wondering whether anyone can recommend a paper cutter/puncher that can make some
from filter paper sheet. I seem to remember the Gatan CP3 comes with one (if I'm not wrong, long time no see)...<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Any advice?<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Best,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Paolo <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:#606060">------------------------------------------------</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:#606060">Paolo Swuec, PhD<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:#606060">Cryo-Electron Microscopy Lab
</span><span style="font-family:"Segoe UI Symbol",sans-serif;color:#606060">❆</span><span style="font-family:"Arial",sans-serif;color:#606060"> Facility Manager<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:#606060">Dep. BioSciences<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:#606060">University of Milano<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:#606060">Via Celoria 26 - 20133 (Italy)<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:#606060">Tel. +39 02 503 147 78<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:#606060"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:#606060">Follow on twitter @PS_CryoEM<o:p></o:p></span></p>
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