[3dem] Screening lamella using Glacios

[Prerana Gogoi]

 Hello All,

I'm just getting started with tomography and would greatly appreciate
input.  I've been fortunate to make several FIB-milled lamellas with a
thickness in the range of 200-300 nm. Unfortunately, getting time on an
ideally configured Krios is difficult and, instead, I might be able to get
time on our Glacios.
My question has to do with whether anyone out there has done screening of
lamella on a Glacios with a K3 camera. I'm concerned that given the fact
that the Glacios operates at 200 keV and does not have an energy filter, my
imaging will not be particularly informative.  If anyone has used a
Glacios/K3 combination to screen lamellas of similar thickness, any
suggestions/comments will be of great help.
Thanks!

Prerana


-- 
*Prerana Gogoi*
*Postdoctoral fellow*
*Gouaux Lab*
*Vollum Institute, OHSU*

*Portland, OR 97239*
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