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<font size="3" face="Calibri,Helvetica,sans-serif,Helvetica,EmojiFont,Apple Color Emoji,Segoe UI Emoji,NotoColorEmoji,Segoe UI Symbol,Android Emoji,EmojiSymbols" color="black"><span style="font-size:12pt" id="gmail-divtagdefaultwrapper"><font color="black"><font size="3" face="Calibri,Helvetica,sans-serif,Helvetica,EmojiFont,Apple Color Emoji,Segoe UI Emoji,NotoColorEmoji,Segoe UI Symbol,Android Emoji,EmojiSymbols" color="black"><span style="font-size:12pt" id="gmail-divtagdefaultwrapper"><div style="margin-top:0px;margin-bottom:0px">Hello All,</div><div style="margin-top:0px;margin-bottom:0px"><br></div>
<div style="margin-top:0px;margin-bottom:0px">I'm just getting started with
tomography and would greatly appreciate input. I've been fortunate to
make several FIB-milled lamellas with a thickness in the range of
200-300 nm. Unfortunately, getting time on an ideally
configured Krios is difficult and, instead, I might be able to get time on
our Glacios. <br></div><div style="margin-top:0px;margin-bottom:0px"> My question has to do with whether anyone out there has
done screening of lamella on a Glacios with a K3 camera. I'm concerned that
given the fact that the Glacios operates at 200
keV and does not have an energy filter, my imaging will not be
particularly informative. If anyone has used a Glacios/K3 combination
to screen lamellas of similar thickness, any suggestions/comments will
be of great help.<br>
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<div>Thanks! <br></div><div><br></div><div>Prerana</div></span></font></font></span></font><br><br><div>-- <b><br></b></div><div><b>Prerana Gogoi</b></div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div dir="ltr"><div><b>Postdoctoral fellow</b></div><div><b>Gouaux Lab</b></div><div><b>Vollum Institute, OHSU</b></div><div><b>Portland, OR 97239<br></b></div></div></div></div></div></div></div></div></div>