[3dem] [ccpem] Saving a dataset normalised with a bad gain ref

[Marin van Heel]

Dear Pranav Shah

Our camera normalisation program should be able to correct your "screwey
gain reference" treated data set...   It improves even on "correctly" gain
corrected data sets!
The condition is that the dataset should be large enough (> ~1000 movie
frames).  Moreover the "screwey gain reference", that you corrupted your
data with, should not contain anomalities, like both negative and positive
values, or undefinined/overflow floating point values.   That being the
case you should able to salvage the data with our program.
Cheers
Marin

For details see: https://www.nature.com/articles/srep10317


On Thu, Mar 14, 2019 at 5:27 AM Pranav Shah <p.shah.lab at gmail.com> wrote:

> Dear Community,
>
> Due to an inadvertent mistake I ended up collecting a dataset
> normalised with a screwey gain ref and I was wondering if there was an
> in silico way to fix this mistake. Has anyone had a similar problem
> and managed to save their precious data?
>
> Looking forward to your suggestions.
> Cheers,
> Pranav
> --
> Pranav Shah
> Postdoctoral Research Fellow.
>
> Hogle Lab
> Department of Biological Chemistry and Molecular Pharmacology,
> Building C2 - First Floor
> 240 Longwood Avenue
> Boston, MA 02115
> (617) 432-4360 (fax)
> (617) 432-3839 (lab)
>
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