[3dem] side entry holder for autogrids?

[Haifeng He]

I just tried what you said in the loading station only and it worked! The
shutter also closes nicely. Great idea! Will still need to see if the drift
rate is affected at Cryo temperature.

Haifeng

On Tue, Jun 25, 2019 at 3:49 PM Michael Elbaum <
michael.elbaum at weizmann.ac.il> wrote:

> Regarding my earlier post about a side entry holder for autogrids, here's
> a simple hack. The autogrid cassette sits comfortably in the 626-70 holder
> tip with the c-clip facing upwards. The Gatan clip ring then fits in nicely
> upside down, like a top hat, and snaps into place with the autogrid trapped
> underneath. I tried this at room temperature and couldn't shake or tap it
> out. We tested in cryo and the grid remained vitrified. There's some
> restriction of the area accessible for tomography that might be remedied
> with a larger hole in the clip ring.
>
> Try at your own risk, etc etc, but it seems to work.
>
> Michael
>
>
> ------------------------------
> *From:* 3dem [3dem-bounces at ncmir.ucsd.edu] on behalf of Wim Hagen [
> hagen at embl.de]
> *Sent:* Monday, June 17, 2019 19:16
> *To:* 3dem at ncmir.ucsd.edu
> *Subject:* Re: [3dem] side entry holder for autogrids?
>
> Hi Michael,
>
> I’ve discussed this with Chris Booth from Gatan some time ago, a multiple
> cartridge side entry holder will for sure also help a lot of people with
> side-entry screening systems, preparing for later data collection  in
> central facilities. With other people’s ideas for cheap side-entry low-kv
> screening tools, this could be quite a market.
>
> Best,
>
> Wim Hagen
> EMBL Heidelberg
>
> On 17. Jun 2019, at 18:00, Michael Elbaum <michael.elbaum at weizmann.ac.il>
> wrote:
>
> Hi all,    wondering if anyone has heard/thought/dreamed of a side entry
> holder for clipped grids? This would be very useful in mixed company (of
> microscopes, of course), and could enable analysis of grids post-tomography
> by non-standard methods such as EDS. Perhaps someone has experience in
> modifying a 626 tip for the purpose?
> regards,
> Michael
>
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20190625/ec68c71a/attachment.html>