[3dem] 3D printed cryo-grid boxes

[John Crum]

Davide,

I have modeled and printed cryo grid boxes. I have not used them, though. I decided not to pursue making and selling them because the cost was about the same as the commercially available ones (about USD 9). I'm not sure that the plastic from any 3D printer type would stand up to LN2 temperatures, over the long run. I do make and sell a manual plunge freezing workstation. You can see it on the 3D printer service "Shapeways" at:

https://www.shapeways.com/product/HZSNAVE4B/plungefreezeinsert-base-v5-1-3

Let me know if you have any questions...

jc


John Crum
JC Designs/California


> On June 10, 2019 at 2:22 AM Davide Zabeo <davide.zabeo at gu.se> wrote:
> 
> 
>     Dear all,
> 
> 
>     In our lab we would like to try 3D-printing cryo-boxes to store plunge frozen cryo-grids (attached photo as reference).
> 
> 
>     Has anyone tried this before? Does anyone have a blueprint of it?
> 
> 
>     Any help is appreciated! Thank you.
> 
> 
>     Best wishes,
> 
> 
>     Davide Zabeo
> 
>     PhD student
> 
>     Johanna Höög Lab
> 
>     Department of Chemistry and Molecular Biology
> 
>     University of Gothenburg
>     Visiting Address: Medicinaregatan 9 C, 41390 Göteborg 
>     Postal Address: Box 462, 40530 Göteborg SWEDEN
> 
 

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