[3dem] PhD position

[Cristina Paulino]

Dear EM community, 

I have a PhD position available to join my energetic lab at the University of Groningen, NL

My group is focused in understanding the structure-function relationship of membrane transporters. Here we employ mostly single-particle cryo-EM which we complement with the biochemical characterization of the target protein. 

We are equipped with an in-house Talos Arctica (auto-loader, K2, energy filter and phase plate), which has proven to be working beautifully and provides super nice high-resolution data of our small membrane proteins (115-250 kDa, low or none symmetry, see some references below). As the head of the cryo-EM unit, members of my (small) group have an exceptionally high access to the microscope and will be thoroughly trained. If required we also have access to two Titan Krios at NeCEN (the Dutch EM facility in Leiden).  

The University of Groningen is continuously ranked among the best 100 universities, is very international, offers excellent working conditions and Groningen itself has a vibrant student city atmosphere. 

I am looking for someone that has already some experience in working with membrane proteins (expression, purification and biochemical characterization) and/or electron microscopy. 

Should you have interest please contact me by email with your CV (including skills and expertise), a motivation letter and at least two contacts for reference. The position would need to be filled as soon as possible. 


Best, 
Cristina


References:
1.	Alvadia, C. et al. Cryo-EM structures and functional characterization of the murine lipid scramblase TMEM16F. Elife 8, 213 (2019).
2.	Kalienkova, V. et al. Stepwise activation mechanism of the scramblase nhTMEM16 revealed by cryo-EM. Elife 8, 213 (2019).
3.	Stock, C. et al. Cryo-EM structures of KdpFABC suggest a K+ transport mechanism via two inter-subunit half-channels. Nat Comms 9, 4971 (2018).
4.	Garaeva, A. A. et al. Cryo-EM structure of the human neutral amino acid transporter ASCT2. Nat Struct Mol Biol 25, 515–521 (2018).
5.	Paulino, C., Kalienkova, V., Lam, A. K. M., Neldner, Y. & Dutzler, R. Activation mechanism of the calcium-activated chloride channel TMEM16A revealed by cryo-EM. Nature 552, 421–425 (2017).



________________________________________________________________________

Dr. Cristina Paulino
Assistant Professor 
Head of the cryo-Electron Microscopy Unit
Department of Structural Biology and Enzymology

Groningen Biomolecular Sciences and Biotechnology Institute (GBB)
Faculty of Science and Engineering, University of Groningen
Nijenborgh 7, 9747 AG Groningen, The Netherlands
Room 5171-0054
Phone: +31 50 363 34 02 
Email: c.paulino at rug.nl 

ORCID: 0000-0001-7017-109X
https://twitter.com/Paulino_Lab
https://www.rug.nl/research/electron-microscopy <https://www.rug.nl/research/electron-microscopy>
http://www.membraneenzymology.com <http://www.membraneenzymology.com/>
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