[3dem] LN2 supply?

[wangpy]

Hi, Bridget,


Cryo-EM facility at SUSTech has been using pipe to fill in its 6 Krios and other instrument with total 9 filling points. A design term for our system only has experiences for industry and sysnchrotron.  Our experiences so far are as:
1, Cost for LN2 is high than use tank directly. This is because every time filling in LN2 to any scope's buffer tank, all pipe have to be cold down. Even installed an isolate valve for all filing points, it still needs to cool down all pipe when a far filling point needs to be used. 
2, LN2 vaporisation is a big issue when filling in tank take place. For such large amount of N2 gas it raises a serious safety issue in the room.  
3, The rate of LN2 evaporation of pipe has to be considered when these pipe have been cold down for all time, especially using a fairly length pipe.
4, It is very time consuming to cool down if pipe do not keep in LN2 temperature all time.
5, The noise is very loud during the pipe cooling down cos N2 gas has to be released.  
We are now modifying our system. Hopefully, these issues will be resolved.
Best.
Peiyi


------------------
Peiyi Wang

SUSTech CryoEM Centre  

Southern University of Science & Technology

Xueyuan Rd, Xili, Nanshan district,

Shenzhen, China, 518055

Tel: (+86) 755 8801 8486 

Email: wangpy at sustc.edu.cn

 


 
 
 
------------------ Original ------------------
From:  "Bridget Carragher"<bcarr at nysbc.org>;
Date:  Sat, Jan 19, 2019 01:27 AM
To:  "3dem"<3dem at ncmir.ucsd.edu>; 

Subject:  [3dem] LN2 supply?

 
Hello all,
We are building a new microscope suite for 3 Krios to add to the 3 we already have.  As everyone who does this will know, managing the LN2 for these instruments becomes quite a burden in terms of swapping tanks, and managing storage and exchange of these tanks.  So we are considering piping LN2 directly into the rooms.  Can anyone with experience please comment on:
- Costs of LN2 when considering that the pipes needs to be cooled down prior to fill and the pipe runs are fairly lengthy from the central storage tank to the rooms.
- Safety concerns in terms of filling accidents in the room displacing oxygen; is this a major problem compared to the 240L tanks
- Concerns that if anything goes wrong with the central storage unit all 6 instruments would go down
- Anything else?
If people want to send their comments directly to me I will collate and share with the list after a few days.
Thanks,
Bridget

 ---------------------------------------------------------------
Bridget Carragher
Simons Electron Microscopy Center
New York Structural Biology Center
89 Convent Avenue, New York NY 10027 
(212) 939-0660;  bcarr at nysbc.org
National Resource for Automated Molecular Microscopy
http://nramm.nysbc.org
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