[3dem] about Cryo-FIB vibration guideline

[Ben Engel]

Hi Chi-yu,

The cryo-stage needs to be really stable and free of vibrations, especially
during fine milling.  The consequence of too much vibration is that it will
be difficult to get your lamellas nice and thin (100-150 nm) without losing
the lamella completely.

I'm not sure how much vibration is tolerable, but I would prioritize having
as little as possible.  That said, I don't think we've done anything
special with our FIBs to minimize vibration, other than placing them on the
ground floor.  They aren't sitting on an isolated slab or anything like
that.  Tell your neighbors to quit their jackhammering.

Best,
Ben

On Thu, Jan 10, 2019 at 7:31 AM fuchiyu <fuchiyu at gate.sinica.edu.tw> wrote:

> Dear 3DEM community,
> We plan to set up Cryo-FIB application in Academia Sinica. FEI/Thermo has
> quite a stringent vibration guideline for Aquilos (VC-E to VC-G). We wonder
> what the setup of other Cryo-FIB sites is and what the possible
> consequences are if we fail to meet the requirement.  Thanks in advance for
> your feedback.
> Best
> Chi-yu
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