[3dem] unmasked FSC different

Takanori Nakane tnakane at mrc-lmb.cam.ac.uk
Mon Feb 11 11:24:31 PST 2019


Hi,

Didn't you use non-converged half maps (i.e. 
run_itNNN_halfX_class001_unfil.mrc)
for PostProcesss?

Best regards,

Takanori Nakane

On 2019/02/11 18:57, Brenda Gonzalez wrote:
> Hello,
> 
> I was wondering if anyone has noticed issues with their FSC results using Relion.
> 
> For a while, I have been getting results where my unmasked FSC curve floats above 0. However, when I looked at the model.star file FSC data and plotted that manually, the unmasked FSC curve actually appears to reach 0.
> 
> Does anyone know what could be causing this or how I could fix it?
> 
> Thank you,
> 
> 
> 
> Brenda Gonzalez
> 
> Department of Biological Sciences – Purdue University
> 
> e: gonzalb at purdue.edu
> 
> 
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
> 



More information about the 3dem mailing list