[3dem] Cryoplunge 3 question

Matthias Wolf matthias.wolf at oist.jp
Thu Feb 7 05:02:51 PST 2019

Hi David,

we also have a CP3 and I have never seen this problem.
I would check that there is sufficient N2 pressure. The mechanism is pneumatic. At low pressure it can get stuck or not move all the way. You could try to increase the gas pressure at your N2 cylinder regulator and check for any leaks at the fittings.

Best regards,
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Morgan, David Gene <dagmorga at indiana.edu>
Sent: Thursday, February 7, 2019 7:01 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Cryoplunge 3 question


    We are currently demo'ing a Gatan Cryoplunge 3 (CP3) freezing robot.  From time to time (and randomly as far as we can tell), there is a significant delay between the time the start button is pushed and when the blotters actually close.  Since the countdown for blotting time starts when the button is pushed, the delay causes the actual blot time for that particular grid to be too short.  Does anyone else who has a CP3 see this issue, and if so, is there anything that can be done about it?  Thanks in advance.

    politics is more difficult than physics.
                                             A. Einstein

            David Gene Morgan
        Electron Microscopy Center
             047D Simon Hall
             IU Bloomington
          812 856 1457 (office)
          812 856 3221 (3200)
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