[3dem] Cryoplunge 3 question

Grassucci, Robert rg2502 at cumc.columbia.edu
Wed Feb 6 15:30:09 PST 2019

Hi David,
Not much experience with the cryoplunge 3 but when we got the modification from cryoplunge 2?  to what they called gentleblot the actual plunging pistons have a dial to increase or decrease the speed of the blotting pistons.  There is also a setting internally that the Gatan service person can set to tweak the amount of pressure delivered to these pistons.  We have seen this behavior before but it seems to behave better with use.  Of course saying that most of our users use the Vitrobot.  Good luck.

-----Original Message-----
From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Morgan, David Gene
Sent: Wednesday, February 06, 2019 5:01 PM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Cryoplunge 3 question


    We are currently demo'ing a Gatan Cryoplunge 3 (CP3) freezing robot.  From time to time (and randomly as far as we can tell), there is a significant delay between the time the start button is pushed and when the blotters actually close.  Since the countdown for blotting time starts when the button is pushed, the delay causes the actual blot time for that particular grid to be too short.  Does anyone else who has a CP3 see this issue, and if so, is there anything that can be done about it?  Thanks in advance.

    politics is more difficult than physics.
                                             A. Einstein

            David Gene Morgan
        Electron Microscopy Center
             047D Simon Hall
             IU Bloomington
          812 856 1457 (office)
          812 856 3221 (3200)
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