[3dem] Data storage and compression

[Takanori Nakane]

Hi,

> (1) I think libtiff has supported building with Zstandard support for a 
> bit (though it is not a common compilation option).  My guess is 
> TIFF+ZStandard will do quite a bit better in the general-case 
> compression dept. than LZW.  Does anyone expect that software packages 
> would automatically work with such images if I built libtiff with 
> Zstandard and linked against it?  I suppose I could just try it, but 
> have not had the chance yet…

RELION does not assume anything about TIFF compression.
As long as the linked libtiff supports it, it should work.

> (2) For cameras like the F3 that output movies with gains pre-applied- 
> it would seem some amount of lossy compression is required to achieve 
> reasonable compression savings- has anyone looked at using 
> “error-bounded” lossy compression algorithms in this use case?  My very 
> naive attempt at quantizing the pixel values (drop 5 LSB) was promising 
> but needs a lot more characterization:

This probably depends on how close you are to the physical Nyquist.

Best regards,

Takanori Nakane