[3dem] Data storage and compression

[Ali Punjani]

Hi Matthew,

In cryoSPARC, LZW compressed tiff files are handled natively and
decompressed in parallel on the fly as needed. They are never stored in
decompressed form, so the original tiff files (created by IMOD, serialEM,
etc) are retained throughout a project. This is a very common workflow,
though as noted, for effective tiff compression the movies should not be
gain corrected - the gain reference must be stored separately and provided
at import time.

Ali

On Thu, Aug 29, 2019 at 3:19 PM Matthew H. Cahn <mcahn at princeton.edu> wrote:

> Is it possible to convert mrc files to compressed tiff immediately after
> data collection, and use that compressed tiff for all the downstream
> processing?  For example, if I compress the mrc files from our Krios to
> tiff with LZW (lossless) compression, using IMOD’s mrc2tif, can motioncor2,
> RELION, CryoSPARC, etc. handle that format and keep everything compressed
> throughout the process?
>
> — Matthew
>
>
> *||  Matthew Cahn  |  Linux Administrator  |  Dept. of Molecular Biology /
> Research Computing  |  Princeton University  |  (609) 258-5404  |
>  mcahn at princeton.edu <mcahn at princeton.edu>  || *
>
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