[3dem] Neg-stain CLEM?

[Matthews-Palmer, Teige Rowan Seal]

Dear 3DEMers,

I’d be interested to hear from anyone who has tried, in the absence of a CLEM cryostage, to correlate objects between fluorescent light microscopy and negative-stain TEM.
Any success avoiding complete rearrangement / breaking on removing a coverslip from an EM grid?
Or any success getting a fluorescent signal out from underneath a dried coat of stain…?
Since this is for a bacterial suspension, negative stain is an obvious, but perhaps not the best fixation method.

Thanks for any info.
All the best
Teige

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