[3dem] EMAN2

[Tim Gruene]

Dear Eric,

CCP-EM claim responsible for the MRC format. They provide a python API
for handling files, which might help to convert the tiff-files to an
mrc-file. They might also have some pre-built tools to avoid the python API.

Besst regards,
Tim

On 09/19/2018 09:40 AM, Eric Hanssen wrote:
> Hi all,
> 
> I am trying to convert a bunch of .tif file (each a stack of 25 frames)
> to .mrc files. why would anyone save as tif instead of mrc I beyond me
> but lets not dwelve in this .
> 
> I ran:
> 
> e2proc2d.py *.tiff @.mrc
> 
>  
> 
> but then get this error message:
> 
>  
> 
> 1 images, processing 0-0 stepping by 1
> 
> Traceback (most recent call last):
> 
>   File "/home/bio21em1/EMAN2/bin/e2proc2d.py", line 1158, in <module>
> 
>     main()
> 
>   File "/home/bio21em1/EMAN2/bin/e2proc2d.py", line 583, in main
> 
>     d.read_image(infile, i)
> 
>   File "/home/bio21em1/EMAN2/lib/EMAN2db.py", line 439, in db_read_image
> 
>     return self.read_image_c(fsp,*parms, **kparms)
> 
> RuntimeError: InvalidValueException at
> /home/bio21em1/Downloads/eman2-master/libEM/emdata_metadata.cpp:844:
> error with '0': 'y size <= 0' caught
> 
>  
> 
> Thoughts?
> 
> Regards
> 
> Eric
> 
>  
> 
> *-----------------*
> 
> *Assoc. Prof Eric Hanssen*
> 
> * *
> 
> *Head - Advanced Microscopy Facility ***
> 
> *Honorary Principal Fellow – Department of Biochemistry and Molecular
> Biology*
> 
> 
> Bio21 Molecular Science and Biotechnology Institute
> 30 Flemington Road - The University of Melbourne - Victoria 3010 - Australia
> email: ehanssen at unimelb.edu.au <mailto:ehanssen at unimelb.edu.au>| Office:
> +61 3 83442449 | Microscope: +61 3 83442509
> 
> Web: www.microscopy.unimelb.edu.au <http://www.microscopy.unimelb.edu.au/>
> 
> qqwd
> 
>  
> 
>  
> 
> 
> 
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-- 
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Paul Scherrer Institut
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Tim Gruene
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phone: +41 (0)56 310 5297

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