[3dem] Large image box size in CryoSparc2 and Relion3

[Elena Orlova]

Hi Everybody,

I wonder if you can help me, any suggestions are welcome:

We need to process particles that are big enough and fit only in the 
boxes of the size 900x900. I do not want to coarsen them.

Is there any way I can process them in CryoSparc (ver 2), or Relion 3? 
So far  CryoSparc2 crashes at 2D classification complaining that : 
*cufftAllosFailed*

If I want to have 100  or 20 classes it fails in ~5min, with 5 classes 
it failed after iteration #19. The error message is the same.

Relion 3 was more efficient: running two (2) month for 50 classes  
reaching the iteration #2. Then the guy was mercilessly killed.

I am using 4 GPU with the memory of ~ 12Gb each

Set consists of ~ 35 000 images.

Many thanks in advance. Elena

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Elena Orlova
Crystallography, Institute for Structural and Molecular Biology,
Department of Biological Sciences
Birkbeck,  University of London,
Malet Street,
LONDON  WC1E 7HX
Tel: (+44) (0)20 7631 6845
Fax: (+44) (0)20 7631 6803
e-mail: e.orlova at mail.cryst.bbk.ac.uk

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