[3dem] 626 holder temperature

Thu Mar 8 08:02:05 PST 2018

Dear Reinhard,

You could try attaching a themocouple, like this one (https://www.omega.com/googlebase/product.html?pn=SA1XL-T-72&gclid=EAIaIQobChMIjp-Us4vd2QIVxlcNCh34BgNNEAQYAiABEgLffPD_BwE) to the outside and check what it reads. From experience, it takes a few mins to reach high temps. At least on FEI holders, the box heats up to 100C, not 80C. And we bake it out for ~12hrs before we use it/them.

We also clean our holders, ~1X/month with an orange degreaser (https://www.officesupply.com/cleaning-breakroom/cleaning-janitorial-supplies/cleaners-disinfectants/specialty-cleaners-lubricants/commercial-heavy-duty-citrus-degreaser/p393154.html?ref=%3Dpla&mrkgadid=3174563176&mrkgcl=609&rkg_id=h-9deed3bbbd9dc4a967367bab8b3a5962_t-1520524670&product_id=393154&adpos=1o1&creative=165794894366&device=c&matchtype=&network=s&gclid=EAIaIQobChMIrpfR34vd2QIVTrjACh0N8g7CEAQYASABEgKbcvD_BwE). We do ~10mins with that, then do 10mins of water and finally 10mins in EtOH or IPA, sonicating the tip each time with each solution, from the area where the O-ring is, to the tip. You could try plasma cleaning it (without the clipring) instead. It has helped with a similar issue on our cryo tomo holder. Definitely check the status of the clipring ... it's possible it's worn.

Best of luck and hope this helps,

Mariena

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Reinhard Rachel <Reinhard.Rachel at biologie.uni-regensburg.de>
Sent: Thursday, March 8, 2018 10:42:42 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] 626 holder temperature

dear cryo-holder 626 experts,

another question to this holder.
We have a 626/70 Jeol version (vintage 2012) and were happy, so far.
a closer look (yesterday, after a rest for 3 months) made us at least curious
to look into details and have a careful check (hopefully, not repair?):
a) heating the holder is done with the smart controller set for 36 to 48 hrs,
to 80°C (whether good or not? remarks are welcome). How to check whether this
temperature is really set? a digital thermometer - inserted into the dewar and
touching the metal part, the bottom of the dewar - reads something like 41 C
(set at 70 C : about 36-38 or so) ... hmm. This means either, the temperature
meter of Gatan is "somewhere else" (likely the case; everything ok??), or the
temperature is not reached, at all. Or anything else?
b) the clipring - when inserted - is rather loose, and not as tight as I
thought it was; or, it should be. "It does not fit any more" - but is this the
only explanation? are the metal parts holding the clipring 'bent'? we will try
the other cliprings (we have 4 more, to try), but I also would appreciate any
comments from other users, regarding this point.

thank you in advance!
Kind regards,
Reinhard

--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail reinhard.rachel at biologie.uni-regensburg.de
office: VKL 3.1.29

Next microscopy conferences:
- 19th International Microscopy Congress, Sydney; 9-14 Sept 2018
http://imc19.com/
- Microscopy Conference MC2019: 1.-5. Sept 2019 in Berlin
- next Microbiol. conferences:
VAAM - Annual Conf.: 15-18 April 2018 Wolfsburg

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