[3dem] Local resolution image

[Mohsen Kazemi]

Hi Jillian and Reza,

This paper should have some useful answers for you; https://www.ncbi.nlm.nih.gov/pubmed/29395788. It introduces a method to calculate local resolution “MonoRes” by J. Vilas et al (Structure, 2018). They propose a fully automatic and accurate method to determine the local resolution of 3D map. And, the method is available in the Scipion.

Cheers,
Mohsen
--
Mohsen Kazemi, PhD, PEng
Postdoctoral Research Fellow

Department of Biochemistry and Molecular Biology
School of Biomedical Sciences
Faculty of Medicine, Dentistry and Health Sciences
The University of Melbourne
Room 363, David Penington Building (Bio21 Institute), 30 Flemington Rd, Parkville, VIC 3052, Australia

Phone : +61 3 834 40285
Email  : mohsen.kazemi at unimelb.edu.au<mailto:mohsen.kazemi at unimelb.edu.au>
              mohsenkazemi20 at gmail.com<mailto:mohsenkazemi20 at gmail.com>

From: 3dem <3dem-bounces at ncmir.ucsd.edu> On Behalf Of Jillian Chase
Sent: Tuesday, June 12, 2018 7:55 AM
To: Reza Khayat <rkhayat at ccny.cuny.edu>
Cc: 3dem at ncmir.ucsd.edu; CCPEM at JISCMAIL.AC.UK
Subject: Re: [3dem] Local resolution image

Hi All,

I'm also very curious about this- has any standard been implemented? It would be nice to have a general guideline so reported data is more-or-less equally easy to interpret in various papers.

We are in the process of drafting a manuscript and have gone back and forth about how to show local resolution in an intuitive way. Some areas of our map are better or worse than the global resolution. What we've settled on (for now) is a rainbow color spectrum (Chimera) in which the range spans both above and below our global res and we have included the scale next to our map colored by res and labeled with resolution. Is there a universal standard that the community is moving towards for this or are we approaching it correctly (or as best we can) for now?

Any insight would be very useful.

All the best,
Jillian

Jillian Chase
PhD Candidate, Jeruzalmi Lab
CUNY/CCNY

On Jun 11, 2018, at 4:58 PM, Reza Khayat <rkhayat at ccny.cuny.edu<mailto:rkhayat at ccny.cuny.edu>> wrote:
Hi,

Has a standard been established in the field for coloring local resolution maps? If not, are there any suggestions (i.e. FSC resolution is blue, 2x FSC resolution is white, and 4x FSC resolution is red). Thanks.

Best wishes,
Reza

Reza Khayat, PhD
Assistant Professor
Department of Chemistry
City College of New York
85 Saint Nicholas Terrace, CDI 2.318
New York, NY 10031
http://www.khayatlab.org/<http://www.khayatlab.org/>
212-650-6070

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