[3dem] Missing wedge masking in RELION

[Gibson, Kimberley]

>From personal experience, after using RELION to perform subvolume averaging on a structure with an extreme preferred orientation - that even tomography would struggle to overcome - I found that RELION did tend to overcompensate the missing wedge. Upon looking at the 3D FTs of our averaged structure, we noticed that much of the Fourier space was filled in and we were rather optimistic about the results. I would approach these results a little more cautiously now as I believe that RELION was blurring together certain features which are now more resolvable using other programs that do apply wedge masking. This is something that could be discussed for future updates to RELION and incorporated into the pipeline, hopefully.

Sincerest regards,

Kimberley Gibson

Research Associate, Charles Sindelar Lab

Dept. Molecular Biophysics and Biochemistry
Yale University

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Steinar Halldorsson <steinar.halldorsson at crick.ac.uk>
Sent: Wednesday, June 6, 2018 5:32:58 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Missing wedge masking in RELION


Dear Tomography folks


A question regarding sub-tomogram averaging in Relion, how does the software treat the missing wedge? Wishful thinking, but does it in any way automatically apply a mask to the individual particles? Is there a way (or will there be a way in the next versions) to apply such a mask?


Many thanks,

Steinar

The Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a company registered in England and Wales no. 06885462, with its registered office at 1 Midland Road London NW1 1AT
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