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<div style="margin-top:0;margin-bottom:0;">From personal experience, after using RELION to perform subvolume averaging on a structure with an extreme preferred orientation - that even tomography would struggle to overcome - I found that RELION did tend to overcompensate
the missing wedge. Upon looking at the 3D FTs of our averaged structure, we noticed that much of the Fourier space was filled in and we were rather optimistic about the results. I would approach these results a little more cautiously now as I believe that
RELION was blurring together certain features which are now more resolvable using other programs that do apply wedge masking. This is something that could be discussed for future updates to RELION and incorporated into the pipeline, hopefully.
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<div style="margin-top:0;margin-bottom:0;">Sincerest regards,</div>
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<div style="font-family:Tahoma; font-size:13px">Kimberley Gibson<br>
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Research Associate, Charles Sindelar Lab<br>
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Dept. Molecular Biophysics and Biochemistry<br>
Yale University<br>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Steinar Halldorsson <steinar.halldorsson@crick.ac.uk><br>
<b>Sent:</b> Wednesday, June 6, 2018 5:32:58 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] Missing wedge masking in RELION</font>
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<p style="margin-top:0; margin-bottom:0">Dear Tomography folks</p>
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<p style="margin-top:0; margin-bottom:0">A question regarding sub-tomogram averaging in Relion, how does the software treat the missing wedge? Wishful thinking, but does it in any way automatically apply a mask to the individual particles? Is there a way (or
will there be a way in the next versions) to apply such a mask?</p>
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<p style="margin-top:0; margin-bottom:0">Many thanks,</p>
<p style="margin-top:0; margin-bottom:0">Steinar</p>
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